尿D-氨基酸氧化酶的ELISA检测和临床应用  

Enzyme immunoassay of D-amino acid oxidase in urine and its clinical application

作　　者：许迅辉[1] 包林平[2] 吴兆龙[1] 阮国芬 李秀兰[2] 

机构地区：[1]上海医科大学附属中山医院肾内科,上海200032 [2]中国科学院上海细胞生物学研究所

出　　处：《中国实验诊断学》2000年第4期156-157,共2页Chinese Journal of Laboratory Diagnosis

摘　　要：目的为探索尿Ｄ－氨基酸测定的临床意义。方法　采用多克隆的ＩｇＧ片段和生物素－链霉亲和素－过氧化物酶系统测定尿Ｄ－氨基酸的含量。检测了２１例正常人和４４例各种肾脏疾病患者尿ＤＡＯ浓度，同时测定尿Ｎ－乙酸－β－Ｄ－氨基葡萄糖苷酶（ＮＡＧ）和α１－微球蛋白（ＡＭＧ）。结果尿ＤＡＯ在各种肾脏疾病患者中均有不同程度的增高，并显示尿ＤＡＯ与ＮＡＧ和ＡＭＧ有高度相关性（ｒ＝０．７２９，Ｐ＜０．０１，ｒ＝０．６４３，Ｐ＜０．０１）。结论提示尿 ＤＡＯ检测可作为临床判断肾功能不良的监测指标之一。Objective In order to study the clincal value of determination of D-amino acid oxidase in urine. Methods We developed an enzyme immunoassay method for the measurement of urinary concentrations of D-amino acid oxidase (DAO), in which the lgG fraction of a polyclonal antibody and the blotinavidin-peroxidase system were used. The urinary concentration of DAO in 21 healthy subjects and in 44 various renal disorders, the concentration of N-acetyl-beta-D-glucosamindase (NAG) and α1-microglobulin (AMG)in the urine were measured and the histological changes of kidney were prelimillarily investigated. Results The findings show a correlation between urinary DAO and urinary NAG(r=0.729,P<0.01) and AMG (r=0.643,P<0.01). Conclusion DAO was judged to he very useful in monitoring the seventy of renal dysfunction.

关 键 词：D-氨基酸氧化酶 生物素-链霉亲和素酶免疫 尿监测 肾脏疾病 

分 类 号：R692.04[医药卫生—泌尿科学] R446.122[医药卫生—外科学]