荧光定量PCR与RT-PCR技术检测HCV-RNA的比较观察  

Study on amplisensor quantitative PCR and RT-PCR in detecting HCV-RNA

作　　者：林潮双[1] 陈文思[1] 卢建溪[1] 谢俊强[1] 柯伟民[1] 高志良[1] 

机构地区：[1]中山医科大学第三附属医院传染科,广东广州510630

出　　处：《中国实验诊断学》2000年第4期176-178,共3页Chinese Journal of Laboratory Diagnosis

基　　金：卫生部部属重点院校临床重点项目资助!(课题编号:97040232)

摘　　要：目的　比较荧光定量 ＰＣＲ与ＲＴ－ＰＣＲ（定性）检测不同检材中的 ＨＣＶ－ＲＮＡ，评价荧光定量ＰＣＲ技术测定ＨＣＶ－ＲＮＡ水平的价值。方法　采用荧光定量ＰＣＲ及 ＲＴ－ＰＣＲ（定性）技术同时检测了７１例血液透析患者的血清、血浆、淋巴细胞、尿液标本中 ＨＣＶ－ＲＮＡ。结果　血清、淋巴细胞、尿液标本中 ＨＣＶ－ＲＮＡ的荧光定量 ＰＣＲ检出率（４６．３８％、８５．５１％、８０．００％），分别高于相应标本中ＨＣＶ－ＲＮＡ的ＲＴ－ＰＣＲ（定性）阳性率（２４．６４、２８．９９％、２０．００％），有统计学意义（Ｐ＜０．０５）。血清、淋巴细胞中同时检出ＨＣＶ－ＲＮＡ的一致率，荧光定量ＰＣＲ法（３９．１３％）显著高于ＲＴ－ＰＣＲ定性法（８．７０％）（Ｐ＜０．００１）。ＨＣＶ－ＲＮＡ的ＲＴ－ＰＣＲ定性阳性率不随 ＲＮＡ定量水平均高而增高。结论与 ＲＴ－ＰＣＲ（定性）相比，荧光定量ＰＣＲ技术检测Objective The aim of the study is to compare amplisensor quantitative PCR with RT-PCR (qualitative) in detecting HCV-RNA of different specimen and to evaluate the technique of amplisensor quantitative PCR. Methods Serum, urine and lymphocyte of 71 hemodialysis patients were tested for hepatitis C viral RNA by amplisensor quantitative PCR and RT-PCR (qualitative). Results Positive rate of HCV-RNA in seurm, lymphocyte, urine by amplisensor quantitative PCR (46.38%. 85.51%, 80.00%)was signdicanily higher than that by RT-PCR respectively (24.64%, 28.99%, 20.00%)(P<0.05).The rate of simultaneously positive HCV-RNA in serum and lymphocyte was significantly higher by amplisensor quantitative PCR (39.13%) than by RT-PCR (8.70%) (P<0.001). There was no relationship between the positive rate of HCV-RNA by RT- PCR and the quantilative level. Conclusion Compared with RT-PCR, amplisensor quantitative PCR technique has higher sen- sitivity in detecting HCV-RNA.

关 键 词：荧光定量PCR RT-PCR 丙型肝炎 HCV-RNA 

分 类 号：R512.630.4[医药卫生—内科学]