利用LC/MS/MS方法检测人参芦头中人参皂苷Rg1和Re的含量  

Methodological Study on Simultaneous Quantitation of Rg1 and Re in "The Rhizoma of Panax ginseng" by LC/MS/MS

作　　者：吴睿凡[1] 赵松岩[2] 刘运嘉 吴晓霞[4] 彭娟[4] 闫寒[4] 聂颖兰[4] 郭娜[4] 范斌[4] 

机构地区：[1]新疆医科大学,乌鲁木齐830011 [2]沈阳药科大学,沈阳110016 [3]沈阳市药品不良反应和医疗器械不良事件监测中心,沈阳110013 [4]中国中医科学院医学实验中心,北京100700

出　　处：《中国中医基础医学杂志》2012年第10期1136-1136,1139,共2页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金青年基金项目(81001597);中国中医科学院医学实验中心自主课题项目(zz2007002)

摘　　要：目的:建立检测人参芦头中人参皂苷Rg1和Re的LC/MS/MS方法。方法:应用液质联用、C18色谱柱系统,采用梯度洗脱方法,建立人参芦头中人参皂苷Rg1和Re的含量测定方法并进行方法学考察。结果:建立了同时检测人参芦头中人参皂苷Rg1和Re的含量测定方法。结论:该方法准确、简便、快速,可用于人参芦头的质量控制。Objective： To develop a method to determine the contents of ginsengnoside Rg~ and Re in the rhizoma of Panax ginseng by using HPLC/MS/MS. Methods： We use the system including HPLC/MS/MS, C18 HPLC column and a gradient elution program to develop a HPLC/MS/MS method. Results： We develop HPLC/MS/MS of simultaneous quantitation of ginsengnoside RgI and Re in the rhizoma of Panax ginseng. Conclusion ： the accurate, convenient and rapid method has been applied for quality control of the rhizoma of Panax ginseng.

关 键 词：人参芦头 含量测定 人参皂苷RG1 人参皂苷RE 

分 类 号：R285.5[医药卫生—中药学]