血红素氧合酶-1对大鼠脑外伤后细胞凋亡的影响  

Effect of heme oxygenase-1 in the central nervous system after brain trauma

作　　者：刘中洪[1] 冯家龙[1] 蒋涛[1] 冉春雷[1] 

机构地区：[1]武警重庆市总队医院神经外科,重庆400061

出　　处：《重庆医学》2012年第30期3132-3133,3137,共3页Chongqing medicine

摘　　要：目的探讨血红素氧合酶-1(HO-1)对大鼠颅脑外伤后细胞凋亡的作用。方法 72只成年雄性SD大鼠,随机分为血晶素组、生理盐水组和锌原卟啉组。采用液压冲击伤复制颅脑外伤的动物模型,伤后予以45mg/100mg体质量腹腔注射诱导剂血晶素、抑制剂锌原卟啉、生理盐水干预HO-1的表达。SP免疫组织化学法测定HO-1和抗凋亡蛋白(Bcl-2)的表达以及TUNEL法检测凋亡细胞。结果血晶素组HO-1表达较生理盐水组和锌原卟啉组显著增加(P<0.01)。血晶素组Bcl-2表达较生理盐水组和锌原卟啉组显著增加(P<0.01)。同时,血晶素组凋亡指数较生理盐水组和锌原卟啉组显著降低(P<0.01)。结论 HO-1在颅脑外伤中的表达,具有上调Bcl-2的表达,抗细胞凋亡的作用。Objective To explore the roles of heme oxygenase-1（HO-1） on apoptosis in the central nerve cells after Brain Trauma.Methods 72 adult Sprague Dawley male rats were divided in three groups：brain trauma＋hemin group,brain trauma ＋ saline group and brain trauma＋ zinc protoporphyrin group.The rat craniocerebral traumatic model was imitated by the device of hydraulic pressure percussion.To interfere in the expression of HO-1,hemin,zinc protoporphyrin and saline（45 mg/100 mg） were injected into every traumatic rat abdominal cavity respectively after craniocerebral trauma.Every rat was detect Apoptotic index and the expression of heme HO-1 and Bcl-2 by SP immunohistochemistry.Results Expression of HO-1 in hemin group remarkably increased compared with salinegroup rats and zinc protoporphyrin group rats（P〈0.01）,Expression of Bcl-2 in hemin group remarkably increased compared with salinegroup rats and zinc protoporphyrin group rats（P〈0.01）.Apoptotic index of nerve cells in hemin group rats remarkably decreased compared with salinerats and zinc protoporphyrin group.Conclusion The expression of HO-1 after brain trauma can up-regulation expression of Bcl-2 and play antiapoptotic role.

关 键 词：血红素加氧酶-1 脑损伤 细胞凋亡 

分 类 号：R651[医药卫生—外科学]