乳鼠耳蜗单离外毛细胞胞膜Prestin分布的实验研究  

Experimental study the prestin distribution at the membrane of single outer hair cells of cochlea in newborn rat

作　　者：张博[1] 尹时华[1] 

机构地区：[1]广西医科大学第一附属医院耳鼻咽喉头颈外科,南宁530021

出　　处：《重庆医学》2012年第30期3178-3179,共2页Chongqing medicine

基　　金：国家自然科学基金资助项目(30860312);国家博士后基金资助项目(20080440819);广西自然科学基金资助项目(桂科自0728134)

摘　　要：目的探讨乳鼠耳蜗外毛细胞(OHC)细胞膜上动力蛋白Prestin的分布。方法获取7日龄的Wistar乳鼠单离的耳蜗OHC,利用Prestin特异性抗体染色,在荧光显微镜下观察Prestin在OHC细胞膜上的表达与分布。结果单离OHC侧膜存在较为均一的Prestin抗体阳性染色;OHC基底部细胞膜亦见阳性染色,但较弱;侧膜与底端细胞膜结合部位未见阳性染色。结论 Prestin主要分布在Wistar乳鼠耳蜗OHC的侧膜,基底部膜也有少量表达。Ohjective To investigate the Prestin distribution at the membrane of single outer hair cells of cochlea in newborn rat. Methods In this experiment,single cell of outer hair cells was geted from 7-day-old wistar rat,the distribution of prestin was examined by imnmnofluorescenl staining and fluorescence microscopy. Results The staining was expressed on the lateral membrane; the base membrane was also expressed, but weaker; on the bottom side of the membrane and the lateral membrane-binding site, The staining was not expressed. Conclusion Prestin is mainly distributed on the lateral membrane,and basal membrane is also a small amount of expression.

关 键 词：乳鼠 耳蜗 外毛细胞 PRESTIN 

分 类 号：R764[医药卫生—耳鼻咽喉科]