ESR测定生物体内NO自由基的几种方法探讨  

Investigation of methods on detection of nitric oxide in vivo by ESR

作　　者：丛建波[1] 李妍[2] 赵洪涛[3] 吴可 王澍 张建中[3] 孙存普 

机构地区：[1]中国人民解放军军事医学科学院放射医学研究所,北京100850 [2]中国人民解放军北京军区联勤部药品器械检验修理所,北京100071 [3]中国科学技术大学研究生院化学部,北京100039

出　　处：《解放军药学学报》2000年第3期149-151,共3页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金课题!No.39670209

摘　　要：目的 :利用电子自旋共振 (ESR)波谱仪检测一氧化氮 (NO)自由基 ,寻找更好的检测生物体内NO自由基的检测方法。方法 :自旋探针咪唑类氮氧自由基 (IN)、血红蛋白 (Hb)溶液和N -甲基 -D -葡糖胺二硫代甲酸铁 [(MGD) 2 -Fe2 +]捕集生物体内外的NO自由基。结果 :( 1)当IN与NO作用后 ,IN本身信号由五重峰变为九重峰 ,最终变为七重峰。 ( 2 )低温 ( 77K)条件下 ,可检测到大鼠经LPS刺激后的股动脉血中的NO自由基。 ( 3) (MGD) 2 -Fe2 +可捕集到小鼠心、肝、肾中NO自由基。结论 :IN捕集NO特异性强 ,但尚不能用于生物体的NO检测。通过探针结构改造 ,用于生物检测是很有可能的 ;Hb测量条件复杂 ,应用受到限制 ;(MGD) 2 -Fe2 +可用于生物体的检测 。Aim To seek out more easy-going method in detection of nitric oxide (NO) in vivo by using electronic spin resonance (ESR) to measure this nitrogen free radical.Methods Comparing the spin trapping function in vitro and in vivo of some spin probes,such as imidazole analogs nitroxide radicals(IN), Hemoglobin(Hb), N-methyl-D-glucamine dithiocarbamate and reduced iron(MGD) 2-Fe 2+ ).Results (1)The spectrum of IN converted from five-line to nine-line and seven-line at last.(2)The NO stimulated by LPS in the rat lateral arteries could be detected by Hb at 77K.(3)(MGD) 2-Fe 2+ has effect on trapping NO in heart, liver and kidney.Conclusion IN is characterized as trapping NO, but its function of chelator was restricted in vitro.Application of Hb is limited because of its measuring complexity.(MGD) 2-Fe 2+ can be used effectively in detecting NO,which may be widely utilized in the measurement of NO.

关 键 词：一氧化氮 电子自旋共振 ESR 自旋捕集 自由基 

分 类 号：R338[医药卫生—人体生理学] R362[医药卫生—基础医学]