同步硝化反硝化细菌的鉴定与脱氮特性研究  

Identification and Characteristics Analysis of Simultaneous Nitrifying-Denitrifying Bacteria and Nitrogen Removing

作　　者：张巍 

机构地区：[1]辽宁省环境科学研究院国家环境保护村镇污水处理与资源化工程研究中心,沈阳110031

出　　处：《土木建筑与环境工程》2012年第5期136-140,共5页Journal of Civil,Architectural & Environment Engineering

基　　金：国家水体污染控制与治理科技重大专项(2012ZX07208-005);国家"十一五"科技支撑计划(2009BADC2B01;2010BAC68B00)

摘　　要：依据同步硝化反硝化脱氮原理,以生活污水处理厂污泥为种泥,利用15℃低温摇床富集并分离出同步硝化反硝化细菌(SNDB-5),采用传统与现代分子生物学相结合的手段对其鉴定,以确定其分类地位;利用水浴摇床研究SNDB-5对硝化细菌液体培养基NO2--N的去除效果。结果表明:SNDB-5与Pseudomonas mendocina同源性达99.0%,形态特征、革兰氏染色及生理生化与Pseudomonas mendocina最相似,属假单胞菌属;摇床培养反应条件显示,当NO2--N浓度加大到150mg/L时,菌株SNDB-5的活性受到了抑制。Based on the principle of simultaneously nitrifying-denitrifying nitrogen removing,sewage treatment plant wastewater sludge was used as inoculum,and the continuous low temperature shaker was used to enrich the simultaneously nitrifying-denitrifying nitrogen removing bacteria（SNDB-5） at 15℃.Traditional and modern molecular biology methods were adopted to determine the taxonomic status of the isolated bacteria which was named SNDB-5.Water bath shaker was used to analyze the NO2——N removal in the nitrifying bacteria culture medium.The results show that SNDB-5 has 99.0 % homology with Pseudomonas mendocina.Morphological,gram staining and biochemical characteristics are also similar to Pseudomonas mendocina.Incubating reaction conditions in shaker shows that SNDB-5 activity is inhibited when the concentration of NO2——N increases to 150 mg/L.

关 键 词：同步硝化反硝化 16SRDNA序列 好氧脱氮 

分 类 号：X703.1[环境科学与工程—环境工程]