Interaction of Carbofuran and Bovine Serum Albumin by Spectrometry  

作　　者：XU Guang-fu TAN Ya-ya LI Bo 

机构地区：[1]Department of Analytical Chemistry,China Pharmaceutical University [2]Xi'an Charoen Pokohand Pharmaceutical Co.,Ltd. [3]Department of Food Quality and Safety,China Pharmaceutical University

出　　处：《Animal Husbandry and Feed Science》2012年第3期138-141,共4页动物与饲料科学（英文版）

摘　　要：[Objective]The aim was to study the interaction characteristic of bovine serum albumin （BSA） and carbofuran. [ Method]With synchronous fluorescence spectrometry adopted, the interaction of carbofuran and BSA in Tris-HCI buffer system （pH 7.40） was investigated. The binding constants at different temperatures were calculated and the interaction types between carbofuran and BSA were discussed. [ Result] Under normal physiological conditions, higher quenching effect of carbofuran on BSA was electrostatic interaction. The changes of different drug concentrations and temperature proved a static quenching of carbofuran with BSA. The binding constants （KSV） at 25 ℃, 37 ℃ and 50 ℃ were 1.17 × 10^4, 1.07 × 10^4 and 0. 99 × 10^4 L/mol respectively with ratio of carbofuran and BSA at 1 ： 1. [ Conclusion ] The research is of guiding significance for learning transport and metabolism of carbofuran at molecular level.[Objective]The aim was to study the interaction characteristic of bovine serum albumin （BSA） and carbofuran. [ Method]With synchronous fluorescence spectrometry adopted, the interaction of carbofuran and BSA in Tris-HCI buffer system （pH 7.40） was investigated. The binding constants at different temperatures were calculated and the interaction types between carbofuran and BSA were discussed. [ Result] Under normal physiological conditions, higher quenching effect of carbofuran on BSA was electrostatic interaction. The changes of different drug concentrations and temperature proved a static quenching of carbofuran with BSA. The binding constants （KSV） at 25 ℃, 37 ℃ and 50 ℃ were 1.17 × 10^4, 1.07 × 10^4 and 0. 99 × 10^4 L/mol respectively with ratio of carbofuran and BSA at 1 ： 1. [ Conclusion ] The research is of guiding significance for learning transport and metabolism of carbofuran at molecular level.

关 键 词：CARBOFURAN Bovine serum albumin Fluorescence spectrometry INTERACTION 

分 类 号：R115[医药卫生—公共卫生与预防医学]