Separation and Measurement of Three Kinds of Endogenous Hormones in Rhizome of Alhagi sparsifolia  

作　　者：ZHAO Man-li YANG Ying-chun YANG Shi-jie YUAN Zhen-yan 

机构地区：[1]College of Life Science and Technology,Xinjiang University

出　　处：《Animal Husbandry and Feed Science》2012年第3期142-144,共3页动物与饲料科学（英文版）

基　　金：Initial Funding of Doctor in Xinjiang University(7020428027);National Innovation Experiment Program for University Students of China (101075512)

摘　　要：[ Objective] The aim was to establish effective method for endogenous hormone extraction and explore conditions of chromatographic analysis for three endogenous hormones in rhizome of Alhagi sparsifolia. [ Methed ] Activol （GA3 ）, zeatin （ZR） and indole-3-acetic acid （IAA） in rhizome were separated and measured as per RP-HPLC. [Result] The average recovery rates of GA3, ZR and IAA were 98.3%, 90.3% and 101.3%, respectively, indicating that the method is suitable for quantitative analysis with little errors. The chromatographic conditions were as follows： methanol/0. 75% of acetic acid at 45：55 （mobile phase） ; flow speed at 0.7 ml/min; wavelength at 254 nm; column temperature at 25 ℃. [Conclusion] The research preliminarily established HPLC conditions for separation of endogenous hormones in rhizome of Alhagi sparsifolia.[ Objective] The aim was to establish effective method for endogenous hormone extraction and explore conditions of chromatographic analysis for three endogenous hormones in rhizome of Alhagi sparsifolia. [ Methed ] Activol （GA3 ）, zeatin （ZR） and indole-3-acetic acid （IAA） in rhizome were separated and measured as per RP-HPLC. [Result] The average recovery rates of GA3, ZR and IAA were 98.3%, 90.3% and 101.3%, respectively, indicating that the method is suitable for quantitative analysis with little errors. The chromatographic conditions were as follows： methanol/0. 75% of acetic acid at 45：55 （mobile phase） ; flow speed at 0.7 ml/min; wavelength at 254 nm; column temperature at 25 ℃. [Conclusion] The research preliminarily established HPLC conditions for separation of endogenous hormones in rhizome of Alhagi sparsifolia.

关 键 词：Alhagi sparsifolia Endogenous hormones Reversed-phase high performance liquid chromatography （RP-HPLC） 

分 类 号：S548[农业科学—作物学]