火龙果组培增殖快繁技术研究  

Tissue Culture and Rapid Propagation of Hylocereus undatus

作　　者：刘洪章[1] 刘艳军[2] 苗博瑛[1] 

机构地区：[1]天津市林业果树研究所,天津300192 [2]天津农学院园艺系,天津300384

出　　处：《天津农业科学》2012年第5期32-34,共3页Tianjin Agricultural Sciences

摘　　要：为了建立火龙果的组培快繁体系,以火龙果的茎段为外植体,消毒后接种到含有不同浓度的6-BA和IAA的MS培养基上,从中筛选出增殖系数最高的培养基配方;将增殖出的火龙果芽接种到含有不同浓度的6-BA和IAA的MS培养基上,从中筛选出最好的伸长培养基配方;将生长到4cm的火龙果幼苗接种到含有不同生长素的生根培养基上,从中筛选出生根效果最好的培养基配方。试验结果显示,最好的增殖培养基配方是:MS+6-BA3.0mg·L-1+IAA0.5mg·L-1;最好的伸长培养基配方是:MS+6-BA1.0mg·L-1+IAA1.0mg·L-1;最好的生根培养基是:1/2MS+IAA1.0mg·L-1。In order to establish the system of Hylocereus undatus tissue culture and rapid propagation, the stem sections of Hylocereus undatus were disinfected . The buds of Hylocereus undatus were regenerated from the disinfect stem sections on MS medium containing 6-BA and IAA of different concentration. The buds of regeneration were cut down and inoculated on MS medium containing 6-BA and IAA of different concentration for elongation. When the buds for elongation were 4 cm high, they were cut down and inoculated on MS medium containing auxin of different concentration for rooting. The experimental results suggested that the optima1 media for buds regeneration were：MS ＋6 -BA3.0 mg·L -1 ＋IAA0.5 mg·L -1;the optima1 media for elongation were：MS ＋6 -BA1.0 mg·L-1＋IAA1.0 mg·L-1; the optima1 media for rooting were1/2MS ＋IAA1.0 mg·L-1.

关 键 词：火龙果 组织培养 快繁 培养基 

分 类 号：S667.9[农业科学—果树学]