甘蓝型油菜不同发育时期株高QTL的动态分析  

Dynamic Analysis of QTL for Plant Height of Rapeseed at Different Developmental Stages

作　　者：谢田田[1,2] 陈玉波[2,3] 黄吉祥[2] 张尧锋[2] 徐爱遐[4] 陈飞[2] 倪西源[2] 赵坚义[2] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321000 [2]浙江省农业科学院作物与核技术利用研究所,浙江杭州310021 [3]杭州师范大学生命与环境科学学院,浙江杭州310036 [4]西北农林科技大学,陕西杨凌712100

出　　处：《作物学报》2012年第10期1802-1809,共8页Acta Agronomica Sinica

基　　金：国家高技术研究发展计划(863计划)项目(2011AA10A104);浙江省科技厅重大专项(2011C12005);浙江省基金重点项目(Z3100592)资助

摘　　要：利用新版SG遗传图谱和282个SG-DH株系在中国西安、中国杭州和德国哥廷根3个生长环境下8个发育时期测定的株高数据,运用WinQTLCart2.5复合区间作图法以及结合条件遗传分析方法对其进行静态和动态QTL分析。结果显示,来自品种Gaoyou等位基因在PHA3和PHC6两个QTL上同时存在时,可降低株高约20cm;而当植株整合来自冬性品种Sollux的PHA9、PHC1和来自半冬性品种Gaoyou的PHA1、PHA3、PHC6时,株高可相应下降40cm;环境对株高QTL的作用机制影响不大,但不同QTL的基因表达模式不同,存在来自双亲之一的等位基因控制株高和双亲等位基因在不同生长时期交替控制株高2种情况;通常株高QTL在中后期才能被检测到,但基因多在生长最为旺盛的短时期内表达,符合基因表达在先,性状表现在后的规律。解析株高性状在不同发育时期基因的累加效应和特定时段内的净表达效应,对克隆油菜株高基因和指导生产实践均有指导意义。The main objective of this study was to explore the genetic control mechanism of plant height in rapeseed and to reveal its dynamic gene expression in specific growing period. Taking advantage of the updated SG map, we determined phenotypic data of plant height from 282 SG-DH lines in three environments and eight development stages, and analyzed both static and dynamic quantitative trait locus （QTL） with unconditional and conditional mapping approach. The results showed that when alleles from Gaoyou existed in two major QTLs PHA3 and PHC6 synchronously, the plant height could decrease about 20 cm; when alleles of plant height came from Sollux in PHA9 and PHC1, and Gaoyou in PHA1, PHA3, and PHC6, the plants fell 40 cm in height. The mechanism of QTL for plant height was less affected by environment, but various QTLs showed difference in gene expression patterns： plant height was dominated by alleles from one of parents or from two parents by turns at different stages. In general, the QTL for plant height could be detected from the middle to late stages, while the gene expression appeared shortly and only during the most active growing period, in congruent with the rule of ＂trait appearance goes after genetic expression＂.

关 键 词：油菜 株高 发育时期 静态QTL 动态QTL 

分 类 号：S565.4[农业科学—作物学]