花生种子白藜芦醇含量与黄曲霉产毒抗性的关系  

Relationship of Resveratrol Content and Resistance to Aflatoxin Accumulation Caused by Aspergillus flavus in Peanut Seeds

作　　者：王后苗[1] 黄家权[1] 雷永[1] 晏立英[1] 王圣玉[1] 姜慧芳[1] 任小平[1] 娄庆任[1] 廖伯寿[1] 

机构地区：[1]中国农业科学院油料作物研究所,农业部油料作物生物学与遗传育种重点实验室,湖北武汉430062

出　　处：《作物学报》2012年第10期1875-1883,共9页Acta Agronomica Sinica

基　　金：国家公益性行业(农业)科研专项(200903004);国家现代农业产业技术体系建设专项(CARS-14);引进国际先进农业科学技术计划(948计划)(2012-S3)资助

摘　　要：选用抗黄曲霉产毒的花生品种(系)和高产毒品种(系),采用强产毒菌株AF2202人工接种水分吸胀的花生种子,培养7d后,测定接种和未接种的花生种子中白藜芦醇及黄曲霉毒素含量,探讨白藜芦醇与花生种子黄曲霉产毒抗性之间的关系。结果表明,抗黄曲霉产毒花生品种(系)的白藜芦醇含量较高,平均为37.3μgkg–1,高产毒品种含量相对较低,平均为13.3μgkg–1,抗、感品种之间存在显著差异。吸胀处理后抗产毒花生品种(系)白藜芦醇含量提高2.0倍,感病品种(系)仅提高1.6倍,对不同品种(系)处理后的种子再接种,令黄曲霉毒素含量下降37.6%～75.8%,但吸胀处理后抗产毒品种(系)的黄曲霉毒素含量仍低于感病品种(系)。相关分析表明,花生白藜芦醇含量与黄曲霉毒素含量呈显著负相关,并且在离体培养基中添加浓度大于3.0μgmL–1的白藜芦醇可导致黄曲霉菌产毒量大幅下降,说明白藜芦醇对黄曲霉产毒具有抑制作用。Resveratrol is an important component of phytoalexins responding to biotic and abiotic stresses. To investigate the possible influence of peanut resveratrol on aflatoxin production by Aspergillus flavus, we selected eight peanut varieties （lines） resistant or susceptible to aflatoxin accumulation caused by Aspergillus flavus to determine resveratrol content and aflatoxin production. The results showed that the average resveratrol content （37.3 μg kg-1 ） of resistant varieties （lines） was significantly higher than that of susceptible ones （13.3 μg kg-1 ） in naturally dried seeds. Resveratrol accumulation amount increased by twice in imbibed resistant peanut seeds, and by 1.6 flod in those of the susceptible ones, and the consequent aflatoxin content declined in the imbibed seeds from 37.6% to 75.8%. However, the resistant varieties （lines） had lower aflatoxin content compared with the susceptible seeds in the imbibition treatments. Resveratrol content in peanut seeds was negatively correlated with aflatoxin production. Trials in vitro also demonstrated that resveratrol could inhibit aflatoxin production in medium. It was suggested that resveratrol is one of the phytoalexins highly related to resistance to aflatoxin production in peanut seeds. As resveratrol has been reported to have certain functions in human health protection, increased resveratrol in peanut would enhance food safety of peanut products and contribute to consumer’s health.

关 键 词：花生 白藜芦醇 黄曲霉产毒抗性 

分 类 号：S565.2[农业科学—作物学]