高效液相色谱法测定牛白藤中齐墩果酸和熊果酸含量  

Determination of oleanolic acid and ursolic acid in Hedyotis hedyotidea by HPLC

作　　者：沈守仁 陈少锋 

机构地区：[1]桂林天和药业股份有限公司,广西桂林541001 [2]广西壮族自治区民族医药研究院,广西南宁530001

出　　处：《现代医药卫生》2012年第20期3067-3068,共2页Journal of Modern Medicine & Health

摘　　要：目的建立以高效液相色谱法(HPLC)同时测定牛白藤药材中齐墩果酸和熊果酸含量的方法。方法色谱柱为Diamonsil C18(4.6 mm×250 mm,5μm),流动相为甲醇∶0.1%磷酸(80∶20),流速为1.0 mL/min,柱温为30℃,检测波长为210 nm。结果齐墩果酸和熊果酸分别在0.091 0～0.910 0μg(r=0.999 8)和0.084 6～0.821 8μg(r=0.999 7)呈良好线性关系,二者平均加样回收率分别为99.91%、98.38%,相对标准偏差(RSD)分别为2.5%、2.2%。结论高效液相色谱法简便、准确、可靠,可用于牛白藤的质量控制。Objective To establish a HPLC method for simultaneous determination of oleanolic acid and ursolic acid in Hedyotis hedyotidea.Methods The analytical column was Diamonsil C18 column（4.6 mm×250 mm,5μm）.The mobile phase consisted of methanol-0.1% phosphoric acid（80 ∶ 20）.The flow rate was 1.0 mL/min,the column temperature was set at 30 ℃ and the detection wavelength was 210 nm.Results The calibration curves of oleanolic acid and ursolic acid showed good linearity in the range of 0.091 0-0.910 0 μg（r=0.999 8） and 0.084 6-0.821 8 μg（r=0.999 7）,respectively.Their average recoveries were 99.91% and 98.38%,RSD 2.5%（n=6）and 2.2%（n=6）,respectively.Conclusion The established method is simple,accurate and reliable,which can be used for the quality control of Hedyotis hedyotidea.

关 键 词：植物 药用 牛白藤 齐墩果酸/分离和提纯 熊果酸/分离和提纯 色谱法 高压液相 

分 类 号：R282.71[医药卫生—中药学]