PCOS患者血清及卵泡液中SCF、TGF-βl水平与PCOS发病及IVF结局的关系  

Correlationship between SCF,TGF-βl level In serum and follicular fluid with PCOS pathogenesis and IVF outcome In PCOS patients

作　　者：孙丽君[1] 孙慧霞[1] 钱秋然[1] 岳丽芳[1] 王兴玲[1] 管一春[1] 李薇薇[1] 娄华[1] 

机构地区：[1]郑州大学第三附属医院生殖中心,450052

出　　处：《中国优生与遗传杂志》2012年第10期111-113,120,共4页Chinese Journal of Birth Health & Heredity

摘　　要：目的探讨多囊卵巢综合征(PCOS)患者血清及卵泡液中干细胞因子(SCF)、转化生长因子βl(TGF-βl)水平与PCOS发病及体外受精-胚胎移植(IVF)结局的相关性。方法选择在郑州大学第三附属医院生殖中心就诊的行IVF的不孕患者79例,PCOS长方案组31例,PCOS微刺激方案组23例,另非PCOS患者长方案25例作为对照组;采用酶联免疫吸附试验(ELISA)检测79例患者HCG日血清及取卵日卵泡液内SCF、TGF-βl水平,用化学发光法测定PCOS患者口服避孕药前后月经第3天基础内分泌值,比较3组患者的年龄、不孕年限、基础内分泌、口服避孕药后基础内分泌的变化;比较PCOS组与对照组患者血清及卵泡液内SCF、TGF-βl水平的差异及上述患者妊娠组与未妊娠组血清及卵泡液内SCF、TGF-βl水平的差异。结果 1.PCOS组在应用口服避孕药后月经第3天血清LH、LH/FSH、T均明显降低,与对照组比较差异无统计学意义(P>0.05)。2.PCOS组卵泡液及血清中的SCF、TGF-βl水平高于对照组,差异有统计学意义(P<0.05)。3.PCOS患者血清和卵泡液内的SCF、TGF-βl水平妊娠组显著高于未妊娠组(P<0.05);对照组患者血清和卵泡液内的SCF、TGF-βl水平妊娠组也明显高于未妊娠组(P<0.05)。结论 1.口服避孕药有利于改善PCOS患者内分泌环境。2.SCF及TGF-βl可能在PCOS的发生发展中起重要作用。3.IVF患者卵泡液内高水平的SCF及TGF-βl可能有利于卵母细胞的成熟、胚胎的发育及胚泡的着床。Objective：To study the correlationship between stem cell factor（SCF） and transforming growth factor βl（TGF-βl） level in the serum and follicular fluid with the polycystic ovary syndrome（PCOS） pathogenesis and in vitro fertilization-embryo transfer（IVF） outcome in PCOS patients.Methods： All the seventy-nine infertile patients who underwent IVF were divided into three groups：the groups of long-term protocol（31 cases）and mild-stimulation protocol（23 cases） in polycystic ovarian syndrome patients and the control group（25 cases,long-term protocol in non PCOS patients）.The age,duration of infertility,basal hormone levels of natural menstrual cycles and the cycles after taking in oral contraceptives,IVF/ICSI-ET outcome and SCF,TGF-βl levels in the serum and follicular fluid were compared among the 3 groups.SCF,TGF-βl levels in the serum and follicular fluid also were compared between the pregnancy group and the non-pregnant group.Results： 1.The levels of basal LH,LH/FSH and T were significantly higher in the first two groups than the last group（P﹤0.05）;The levels of LH,LH/FSH,T in the groups of PCOS patients decreased obviously after the use of oral contraceptives,there were no significant differences between the PCOS groups with the non PCOS group.2.SCF,TGF-βl levels in follicular fluid and serum in the PCOS groups higher than control group,the difference was statistically significant（P〈0.05）.3.In PCOS patients,SCF,TGF-βl level in pregnant group was significantly higher than non pregnant group（P〈0.05）.in non PCOS patients,SCF and TGF-βl level was also significantly higher in pregnant group than non pregnancy group（P〈0.05）.Conclusions：1.Oral contraceptives improve PCOS patients endocrine environment.2.SCF and TGF-βl may play an important role in the pathogenesis of PCOS.3.High levels of SCF and TGF-βl in follicular fluid may be beneficial to the oocyte maturation,embryo development and blastocyst implantation in IVF patients.

关 键 词：体外受精-胚胎移植 多囊卵巢综合征 卵泡液 SCF TGF-βl 

分 类 号：R711.75[医药卫生—妇产科学]