乙脑病毒SA_(14-14-2)减毒株病毒外源因子污染检测分析  

Adventitious Virus Agent Contamination in Japanese Encephalitis Attenuated Virus Strain(SA 14-14-2)

作　　者：王红[1] 彭维[1] 刘菊[1] 嫣然 屈三甫[2] 郑从义[2] 

机构地区：[1]成都生物制品研究所有限责任公司,成都610023 [2]武汉大学生命科学学院中国典型培养物保藏中心

出　　处：《预防医学情报杂志》2012年第10期768-771,共4页Journal of Preventive Medicine Information

基　　金：国家科技支撑计划(2008BAI66B05)乙脑减毒活疫苗高技术产业化示范工程(发改办高技[2006]2352号)

摘　　要：目的检测乙脑病毒SA14-14-2减毒株的病毒外源因子。方法用乙脑病毒免疫血清(阳性)将乙脑病毒SA14-14-2减毒株中和后,采用中和后的样品分别感染指示细胞(3T3、NRK、C3/36细胞),直接培养观察及红细胞吸附试验;中和后样品感染小鼠细胞系(3T3)培养14d的上清液再接种指示细胞(MRC-5、Vero、BHK21、3T3)培养观察及红细胞吸附试验;同时将流感病毒接种MDCK细胞,作为试验阳性对照。结果乙脑病毒SA14-14-2减毒株中和后的样品接种指示细胞(3T3、NRK、C6/36细胞),直接培养观察7 d、14 d,细胞形态正常,无细胞病变征兆,红细胞吸附试验为阴性;中和后样品感染小鼠细胞系(3T3)培养14 d的上清液再接种指示细胞(MRC-5、Vero、BHK-21、3T3)培养观察7 d、14 d,细胞形态正常,无细胞病变征兆,红细胞吸附试验为阴性。结论乙脑病毒SA14-14-2减毒株中未检测到人源、猴源、鼠源及其他病毒的污染,符合《WHO Technical Report Series,No.910,2002》的质量标准,可安全地用于乙脑减毒活疫苗的生产。Objective To test the adventitious virus agent in Japanese encephalitis attenuated virus strain（SA 14-14-2）.Methods Samples from neutralization of SA 14-14-2 by positive immune serum were selected to infect indicator cells（3T3,NRK,C3/36） and revaccination indicator cells（MRC-5,Vero,BHK21,3T3） from 14-day-cultured supernatant of mouse cell line（3T3）,followed by both culture observation and hemabsorption;MDCK cells inoculated by flu virus were set as positive control.Results Observation at time points of 7 d and 14 d showed normal cell morphology with no cytopathic changes as well as negative hemadsorption outcomes.Conclusion With no adventitious virus detected,SA 14-14-2 meets quality criteria of WHO Technical Report Series,No.910,2002 and can be put into production.

关 键 词：乙脑病毒SA14-14-2减毒株 病毒外源因子 鼠源细胞 蚊子细胞 

分 类 号：R373.3[医药卫生—病原生物学]