血吸虫对吡喹酮抗药性的研究XIIV湖沼型流行区日本血吸虫对吡喹酮的敏感性评价  

Studies on resistance of Schistosoma to praziquantel XIIV Sensitivity of Schistosoma japonicum to praziquantel in marshland and lake endemic regions of Chinese Mainland

作　　者：汪伟[1] 李洪军[1] 梁幼生[1] 曲国立[1] 李幼子[1] 邢云天[1] 钱科[1] 贾悦[1] 杨振坤[1] 王飞[1] 戴建荣[1] 

机构地区：[1]江苏省血吸虫病防治研究所、卫生部寄生虫病预防与控制技术重点实验室,无锡214064

出　　处：《中国血吸虫病防治杂志》2012年第5期505-509,共5页Chinese Journal of Schistosomiasis Control

基　　金：国家科技支撑计划(2009BAI78B06);江苏省卫生厅项目(X200901、X200911、X200912、X201111);江苏省预防医学科研课题(Y201031)

摘　　要：目的比较中国大陆湖沼型流行区日本血吸虫不同分离株对吡喹酮的敏感性,为建立吡喹酮敏感性检测/监测技术提供实验依据。方法从湖南、湖北、江西、安徽和江苏5省湖沼型血吸虫病流行区现场采集感染性钉螺,分离尾蚴,以40条各虫株尾蚴感染小鼠,感染35 d后分为6组,5个用药组分别一次灌服37.5、75、150、300 mg/kg和600 mg/kg吡喹酮,对照组小鼠灌服2.5%Cremophor EL溶液。所有小鼠于感染后50 d解剖,收集小鼠体内成虫,统计虫负荷和减虫率,计算并比较不同分离株血吸虫的吡喹酮半数有效剂量(ED50值)。结果37.5、75、150、300 mg/kg和600 mg/kg吡喹酮一次灌胃给药后,各组小鼠减虫率分别为10.37%～19.81%、23.22%～33.09%、39.25%～49.61%、62.87%～74.44%和91.26%～98.09%。经不同剂量吡喹酮给药后,各组小鼠减虫率差异无统计学意义(P>0.05)。7株日本血吸虫不同分离株的吡喹酮ED50值为134.1～186.7 mg/kg,但各分离株的吡喹酮ED50值差异无统计学意义(P>0.05)。结论中国大陆湖沼型流行区日本血吸虫不同分离株对吡喹酮的敏感性差异无显著性。血吸虫吡喹酮ED50值作为定量指标,能够客观反映血吸虫种群对吡喹酮的敏感性。Objective To compare the sensitivities of different isolates of Schistosoma japonicum in marshland and lake re-gions of Chinese Mainland to praziquantel, so as to provide experimental evidence for establishing the techniques of detecting and monitoring praziquantel sensitivity. Methods Mice were infected with cereariae released from the S. japonicum-infeeted snails collected from the marshland and lake endemic regions of Hunan, Hubei, Jiangxi, Anhui and Jiangsu provinces, grouped, and treated with praziquantel at single oral doses of 37.5, 75, 150, 300 mg/kg and 600 mg/kg, while mice infected but treated with 2.5% Cremophor EL served as controls. The worm burden reductions caused by praziquantel treatment were observed, and the 50% effective dose （ED50 value） was calculated. Results The administration with praziquantel at single oral doses of 37.5, 75, 150, 300 mg/kg and 600 mg/kg achieved worm burden reductions of 10.37%-19.81%, 23.22%-33.09%, 39.25%-49.__61%, 62.87%-74.44% and 91.26%-98.09%, respectively, and no significant differences in worm burden reductions of different isolates of S. japonicum were detected following treatment with different doses of praziquantel （P 〉 0.05）. The praziquantel ED50 values against different isolates of S. japonicum ranged from 134.1 to 186.7 mg/kg, and no significant differences of praziquantel ED50 val-ues were found among different isolates （P 〉 0.05）. Conclusions There were no significant differences of praziquantel sensitivi-ties of different isolates of S. japonicum in marshland and lake regions of Chinese Mainland. Praziquantel ED50 value against sehis-tosomes, as a quantitative index, can truly reflect the sensitivity of sehistosome populations to praziquantel, which can be used to assess the risk of emergence of praziquantel resistance in schistosomes.

关 键 词：日本血吸虫 吡喹酮 敏感性 半数有效剂量(ED50) 湖沼地区 

分 类 号：R383.24[医药卫生—医学寄生虫学]