妊娠大鼠吸入异氟烷、七氟烷对子代海马MAPK信号传导的影响  

Effect of isoflurane and sevoflurane inhalated by pregnant rats on brain development of MAPK signal transduction pathway of progeny rats

作　　者：王宇恒[1] 艾春雨[2] 成永霞[3] 赵宏[1] 王俊科[2] 

机构地区：[1]沈阳医学院奉天医院麻醉科,辽宁沈阳100011 [2]中国医科大学附属第一医院麻醉科,辽宁沈阳100011 [3]牡丹江医学院病理教研室,黑龙江牡丹江1570113

出　　处：《中国热带医学》2012年第9期1052-1054,共3页China Tropical Medicine

基　　金：黑龙江省青年科学基金项目(No.QC2010040)

摘　　要：目的观察大鼠妊娠前后暴露于最低肺泡有效浓度(1MAC)的异氟烷或七氟烷是否会引起子代海马中与MAPK信号转导通路相关的指标的变化。方法大鼠妊娠前后吸入麻醉药模型的建立,Western方法检测子代大鼠海马中p-ERK1/2及PKCα蛋白的表达。结果子代海马p-ERK1/2及PKCα蛋白的表达与对照组相比无明显差异,而母体于妊娠6、10、14和18d暴露于1MAC值的异氟烷或七氟烷,可使子代大鼠0、7、14d海马p-ERK1/2蛋白的表达显著减少(t=2.63,P<0.01),PI组较PS组表达减少(t=2.13,P<0.05),而这种变化于出生后28d消失。结论大鼠妊娠前后暴露于1MAC的异氟烷或七氟烷引起的子代大鼠海马中MAPK信号转导通路相关的指标发生变化。Objective To observe if inhalation of isoflurane and sevoflurane by pregnant rats can effect MAPK signal transduction pathway in progeny rats.Methods Western-blotting was used to detect PKCα、p-ERK1/2 protein expression in P0,P7,P14 and P28 progeny hippocampus.Results The expression of PKCα,p-ERK1/2 protein in progeny rats showed no significant difference compared to the control group.The expression of p-ERK1/2 protein in progeny rats 0,7 and 10 days afte born would be significantly reduced in pregony rats 6,10,14 and 18 days after whose mothers exposed to isoflurane and sevoflurane（t=2.63,P0.01）.PI group showed significant change compared with PS group（P0.05） but the change would disappear 28 days after born.Conclusion The related indicators of MARK singnal parthway of the progeny rats would change if the pregnant rats exposed to isoflurane or sevoflurane.

关 键 词：异氟烷 七氟烷 MAPK信号转导通路 p-ERK1 2 PKCΑ 

分 类 号：R614.1[医药卫生—麻醉学]