白细胞介素-1β基因多态性与新型甲型H1N1流感的关系  

The relationship between single nucleotide polymorphisms of IL-1β gene and susceptibility to influenza A (H1N1) virus

作　　者：杨辉[1,2] 张国良[2] 汪文斐[2] 陈心春[2] 郑学宝[3] 吴爱武[4] 

机构地区：[1]广州医学院,广东广州510182 [2]深圳市第三人民医院,广东深圳518112 [3]广东医学院,广东东莞523024 [4]广州医学院第一附属医院,广东广州510260

出　　处：《中国热带医学》2012年第9期1055-1057,1061,共4页China Tropical Medicine

基　　金：深圳市科技计划项目(No.201002107);东莞市科技计划项目(No.200910815229)

摘　　要：目的探讨白细胞介素-1β（IL-1β）基因单核苷酸多态性（SNP）与新型甲型H1N1流感易感性的关系。方法针对IL-1β基因5’端的4个SNP位点（rs1143623,rs1143639,rs16944,rs3917345）,使用飞行时间质谱分析技术（TOF-MS）对167例H1N1流感组患者和192例健康对照组人群检测其基因多态性。结果rs16944有A和G两种等位基因,H1N1组A等位基因频率为61.9%,对照组为49.5%,差异有统计学意义（χ2=10.761,P=0.001,OR=0.602,95%CI0.444~0.816）;rs1143639有G和A两种等位基因,G等位基因频率在H1N1组中为95.5%,对照组为98.7%,差异也有统计学意义（χ2=6.708,P=0.0096,OR=3.564,95%CI 1.281~9.917）;其余两个位点在两组人群间无统计学意义。结论rs16944和rs1143639位点与新型甲型H1N1流感易感性相关。Objective To explore the association between IL-1β gene single nucleotide polymorphisms and the susceptibility influenza A（H1N1） virus.Methods Four SNPs（rs1143623,rs1143639,rs16944,rs3917345）were collected in IL-1 β gene and genotyped with TOF-MS assay among influenza A（H1N1） groups（n=167） and healthy controls（n=192）.Results rs16944 SNP involved A and G allele.The A allele frequency in H1N1 groups and healthy controls group were 61.9% and 49.5%,respectively,showing significant difference（χ2=10.761,P=0.001,OR=0.602,95% CI 0.444~0.816）;rs 1143639 SNP involved G and A allele,and the G allele frequency in H1N1 groups and healthy controls were 95.5% and 98.7%,also showing significant difference（χ2=6.708,P=0.0096,OR=3.564,95% CI 1.281~9.917）;no significant difference was observed in allele frequency between H1N1 group and healthy control group for other SNPs.Conclusion rs16944 SNP and rs1143639 SNP of IL-1β gene were associated with susceptibility to influenza A（H1N1） virus.

关 键 词：白细胞介素-1Β 单核苷酸多态性 甲型H1N1流感 飞行时间质谱分析技术 

分 类 号：R511.7[医药卫生—内科学]