Isolation and Characterization of Heterotrophic Nitrifying Strain W1  

异养硝化菌株W1的分离和表征(英文)

作　　者：吕永康 王荀 刘博凯 刘玉香 杨晓华 

机构地区：[1]Key Laboratory of Coal Science and Technology,Taiyuan University of Technology [2]Engineering&the Built Environment(EBE),University of Cape Town [3]College of Environmental Science and Engineering,Taiyuan University of Technology

出　　处：《Chinese Journal of Chemical Engineering》2012年第5期995-1002,共8页中国化学工程学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China (51078252);the International Cooperation Projects of Shanxi Province (2010081018);the Natural Science Foundation of Shanxi Province (2010011016-1)

摘　　要：In a high concentration substrate medium, a heterotrophic bacterium with high removal efficiency of ammonium, named W1, was isolated from activated sludge of coking wastewater treatment facility. The bacterium was Gram-negative, rod-shaped, and identified preliminarily as Alcaligenes sp. according to its morphological and physiological properties and its 16S rRNA gene sequence analysis. In the high concentration ammonium medium (400 mg·L 1 4 NH -N), the effects of C source, N source, C/N ratio and initial pH of medium on ammonium removal were investigated in order to determine the optimal condition for strain W1. The maximum ammonium removal was around 95% in 4 days in an improved medium. The production of N 2 gas was examined in a closed system that was full of pure oxygen at the beginning. N 2 gas was detected in the system after 4 days of cultivation, which further testified that strain W1 has heterotrophic nitrification and aerobic denitrification abilities simultaneously.In a high concentration substrate medium, a heterotrophic bacterium with high removal efficiency of ammonium, named W1, was isolated from activated sludge of coking wastewater treatment facility. The bacterium was Gram-negative, rod-shaped, and identified preliminarily as Alcaligenes sp. according to its morphological and physiological properties and its 16S rRNA gene sequence analysis. In the high concentration ammonium medium （400 mg·L 1 4 NH -N）, the effects of C source, N source, C/N ratio and initial pH of medium on ammonium removal were investigated in order to determine the optimal condition for strain W1. The maximum ammonium removal was around 95% in 4 days in an improved medium. The production of N 2 gas was examined in a closed system that was full of pure oxygen at the beginning. N 2 gas was detected in the system after 4 days of cultivation, which further testified that strain W1 has heterotrophic nitrification and aerobic denitrification abilities simultaneously.

关 键 词：heterotrophic nitrification aerobic de-nitrification high concentration ammonium Alcaligenes sp. 

分 类 号：X784[环境科学与工程—环境工程]