Expression Patterns of Nine Ammonium Transporters in Rice in Response to N Status  

作　　者：LI Su-Mei LI Bao-Zhen SHI Wei-Ming 

机构地区：[1]State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences,Nanjing 210008(China) [2]Key Laboratory of Agro-ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Hunan 410125(China)

出　　处：《Pedosphere》2012年第6期860-869,共10页土壤圈（英文版）

基　　金：Supported by the National Natural Science Foundation of China (No. 30800702);the National Basic Research Program of China (No. 2007CB109303);the National Key Technology R&D Program of China (No. 2012BAD15B03)

摘　　要：Nitrogen use efficiency （NUE） was very low in China and a loss of as much as 7070 of the applied nitrogen fertilizers was reported in high-yielding rice fields. In order to investigate the molecular basis of high-affinity ammonium transport or uptake into rice （Oryza sativa L.）, we analyzed the expression profiles of nine ammonium transporters （AMT）, three each of OsAMT1, OsAMT2 and OsAMT3, at two different N requirement stages （young seedling stage and tillering stage） of rice growth as well as the changes in these expression patterns according to external N status using real-time reverse transcription polymerase chain reaction （RT-PCR）. The results suggested that the nine OsAMT genes were expressed in different organs of rice plants, including mature roots, new roots, stems, old leaves and new leaves and that the expression patterns were organ specific and independent of the positions of the corresponding proteins in the phylogenetic tree. OsAMT1;1, 3;2 and 3;3 were expressed in the roots and shoots, primarily old leaves, OsAMT1;2 and 1;3 mainly in the roots, and OsAMT2;1, 2;2, 2;3 and 3;1 mainly in the shoots, primarily in new leaves, and relatively more in the stems than other genes. The expression patterns at the two different N requirement stages were the same; however, at the tillering stage with greater N requirements, the OsAMTs transcript levels were greater than those at the young seedling stage with low N requirements. N starvation for 48 h up-regulated OsAMT1;1, 1;2, 3;1, 3;2, 3;3 and down-regulated OsAMT1;3 mRNA abundance. Following N starvation, NH4＋ and NH4NO3 re-supply down-regulated OsAMT1;2 and 3;3 and up-regulated OsAMT1;3, whereas NO3 re-supply down-regulated OsAMT1;1 and 1;2. These suggested that the organ-specific expression pattern of OsAMT could be regulated by N requirement and external N status.Nitrogen use efficiency (NUE) was very low in China and a loss of as much as 70% of the applied nitrogen fertilizers was reported in high-yielding rice fields. In order to investigate the molecular basis of high-affinity ammonium transport or uptake into rice (Oryza sativa L.), we analyzed the expression profiles of nine ammonium transporters (AMT), three each of OsAMT1, OsAMT2 and OsAMT3, at two different N requirement stages (young seedling stage and tillering stage) of rice growth as well as the changes in these expression patterns according to external N status using real-time reverse transcription polymerase chain reaction (RT-PCR). The results suggested that the nine OsAMT genes were expressed in different organs of rice plants, including mature roots, new roots, stems, old leaves and new leaves and that the expression patterns were organ specific and independent of the positions of the corresponding proteins in the phylogenetic tree. OsAMT1;1, 3;2 and 3;3 were expressed in the roots and shoots, primarily old leaves, OsAMT1;2 and 1;3 mainly in the roots, and OsAMT2;1, 2;2, 2;3 and 3;1 mainly in the shoots, primarily in new leaves, and relatively more in the stems than other genes. The expression patterns at the two different N requirement stages were the same; however, at the tillering stage with greater N requirements, the OsAMTs transcript levels were greater than those at the young seedling stage with low N requirements. N starvation for 48 h up-regulated OsAMT1;1, 1;2, 3;1, 3;2, 3;3 and down-regulated OsAMT1;3 mRNA abundance. Following N starvation, NH+4 and NH4NO3 re-supply down-regulated OsAMT1;2 and 3;3 and up-regulated OsAMT1;3, whereas NO3- re-supply down-regulated OsAMT1;1 and 1;2. These suggested that the organ-specific expression pattern of OsAMT could be regulated by N requirement and external N status.

关 键 词：external N level gene expression growth stage N requirement plant organ 

分 类 号：S511.03[农业科学—作物学] S511