高压电晕电场对黄霉素产生菌诱变效应  

EFFECT OF CORONA DISCHARGE ON Flavomycin PRODUCTING STRAIN

作　　者：谷卓[1] 那日[1] 石薇[1] 徐淑银[1] 

机构地区：[1]内蒙古大学物理科学与技术学院,内蒙古呼和浩特010021

出　　处：《核农学报》2012年第5期740-745,共6页Journal of Nuclear Agricultural Sciences

基　　金：内蒙古自治区科技计划项目(20091502);国家大学生创新性实验计划项目(091012611)

摘　　要：利用高压电晕电场诱变技术对黄霉素产生菌08-28进行微生物代谢产量和活性的研究。结果表明:链霉素对黄霉素产生菌的最低抑制浓度为0.75U/ml;随着电场处理剂量的增大菌种致死率也随之增大,诱变电压为21kV,针尖距离为8mm,诱变时间为4min时是最佳诱变条件,此时致死率在68%,菌落形态呈放射状褶皱。通过筛选,获得8株突变高产菌株,其中产黄霉素能力最高的是原始菌株的1.92倍。Mutagenic effect of corona discharge on the flavomycin producing strain 08-28 was carried out,and the yield and activity of flavomycin were studied.The results showed that the minimum inhibiting concentration of streptomycin for flavomycin producing strain was 0.75U/ml.The lethal rate was increased with the increasing strength of electric field treatment.The optimum condition for mutation was that the mutagenesis voltage 21kV,the tip distance 8mm,the mutagenesis time 4min.On this condition the strain lethal rate was 68% and the typical colony of the mutant was radial and drape.As a result,8 high-yielding strains were obtained,and the highest ability to produce flavomycin was increased by 1.92 times than original strain.

关 键 词：黄霉素产生菌 电晕电场 诱变 

分 类 号：S859.79[农业科学—临床兽医学]