槲皮素长循环纳米脂质体的制备及大鼠体内药动学参数测定  

Preparation and Pharmacokinetic Study Long Circulating Nano-Liposomes of Quercetin in Rats

作　　者：王刚[1,2] 杜士明[2] 常明泉[2] 曾南[1] 叶方[2] 陈永顺[2] 

机构地区：[1]成都中医药大学,610075 [2]湖北医药学院附属太和医院药学部,湖北十堰442000

出　　处：《医药导报》2012年第10期1275-1278,共4页Herald of Medicine

基　　金：湖北省教育厅优秀中青年基金(Q20102110)

摘　　要：目的制备槲皮素长循环纳米脂质体(QUE-CNL),并测定其在大鼠体内的药动学参数。方法采用乳化蒸发-低温固化法制备QUE-CNL;高效液相色谱法测定槲皮素及QUE-CNL在大鼠体内不同时间点的血药浓度,用3p97药动学软件处理数据,计算药动学参数。结果 QUE-CNL在大鼠体内血浆浓度显著高于槲皮素血浆浓度,QUE-CNL药-时曲线下面积较槲皮素大(P<0.01),表观分布容积较槲皮素低(P<0.01),血浆清除率较槲皮素慢(P<0.01)。结论以乳化蒸发-低温固化法制备的QUE-CNL可延长槲皮素在血浆中循环时间。Objective To prepare the long circulating nano-liposomes（QUE-LCL） of quercetin and measure their pharmacokinetic parameters in rats.Methods QUE-LCL was prepared by emulsified evaporation and solidification at low temperature.The blood samples were collected at different time points and concentrations of quercetin and QUE-LCL were measured by HPLC.The pharmacokinetic parameters were calculated with the software 3p97.Results The plasma QUE concentration and area under the curve（AUC） of QUE-LCL in rats were obviously higher than those of quercetin suspension.The volume of distribution（Vd） and plasma clearances of QUE-LCL were lower than those of quercetin suspension.Conclusion QUE-LCL prepared by emulsified evaporation and solidification at low temperature can prolong the circulation of quercetin in plasma,leading to 1ong circulating effects.

关 键 词：槲皮素 长循环纳米脂质体 色谱法 高效液相 药动学参数 

分 类 号：R969.1[医药卫生—药理学]