拉米夫定和干扰素α2b体外细胞毒性与抗乙型肝炎病毒作用比较  

Comparison of Cytotoxicity and Anti-HBV of Lamivudine and Interferon α2b in Vitro

作　　者：马艳[1] 金小宝[1] 黄演婷[1] 卢雪梅[1] 

机构地区：[1]广东药学院广东省生物活性药物研究重点实验室,广州510006

出　　处：《医药导报》2012年第10期1294-1297,共4页Herald of Medicine

摘　　要：目的体外比较拉米夫定和α-干扰素的细胞毒性和抗乙型肝炎病毒作用。方法体外培养L-02和HepG2.2.15细胞,给予不同浓度的拉米夫定和干扰素α2b,作用72 h后,噻唑蓝(MTT)法检测L-02和HepG2.2.15细胞的生存率;收集不同浓度的拉米夫定和干扰素α2b处理的HepG2.2.15细胞上清液,酶联免疫吸附测定(ELISA)法检测HBsAg、HBeAg的分泌。结果拉米夫定对L-02和HepG2.2.15有一定的毒性作用,而干扰素α2b对细胞无毒性作用,甚至能促进正常细胞的增殖;拉米夫定对HBsAg和HBeAg的抑制作用大于干扰素α2b,拉米夫定对HBsAg的抑制率较高,而干扰素α2b对HBeAg的抑制率较高。结论拉米夫定和α干扰素联合使用可提高抗HBV的作用和质量。Objective To compare the cytotoxicity and anti-hepatitis B virus effect of lamivudine and α-interferon in vitro.Methods L-02 and HepG2.2.15 were treated with different concentrations of lamivudine and interferon α2b for 72 h in vitro,the survival rates of L-02 and HepG2.2.15 were assayed by MTT.HbsAg and HBeAg secretion of HepG2.2.15 was detected by ELISA.Results Lamivudine could inhibit L-02 and HepG2.2.15 in a certain extent,while interferon α2b promoted the proliferation of normal cells.Inhibition effect of lamivudine on HBsAg and HbeAg was greater than that of interferon α2b,and HBsAg inhibition rate of lamivudine was higher,while HbeAg inhibition rate of interferon α2b was the higher.Conclusion Combined use of lamivudine and α-interferon can improve the anti-HBV effect and quality.

关 键 词：拉米夫定 干扰素Α2B 细胞毒性 抗HBV 

分 类 号：R978.7[医药卫生—药品] R965[医药卫生—药学]