高效液相色谱法测定伊曲康唑传递体的包封率与含量  

Determination of Content of Itraconazole Transfersomes and Its Entrapment Efficiency by HPLC

作　　者：郑稳生[1] 方夏琴[1] 张宇佳 王璐璐[1] 

机构地区：[1]中国医学科学院北京协和医学院药物研究所,药物传输技术及新型制剂北京市重点实验室,100050

出　　处：《医药导报》2012年第10期1347-1349,共3页Herald of Medicine

摘　　要：目的建立高效液相色谱法测定伊曲康唑传递体的含量及包封率。方法采用C18色谱柱(150 mm×4.6 mm,5μm);柱温:室温;流动相:乙腈-水(65∶35);流速:1 mL.min-1;检测波长:261 nm。甲醇为溶剂,测定传递体中伊曲康唑的含量。结果伊曲康唑在1～100μg.mL-1范围内有良好的线性关系,r=0.999 7;平均回收率为101.9%,RSD为1.7%。结论该方法准确可靠、简单快速,专属性高,可用于伊曲康唑传递体含量及包封率的测定。Objective To establish an HPLC method for determination of content and entrapment efficiency of itraconazole transfersomes.Methods The content of itraconazole was separated with a C18 column（150 mm×4.6 mm,5 μm）.The mobile phase was acetonitrile-water（65：35） at a flow rate of 1.0 mL·min^-1 and itraconazole was detected at 261 nm and room temperature.Results The linear range of itraconazol was 1 to 100 μg·mL-1（r=0.999 7）.The average recovery was 101.9% and RSD was 1.7%.Conclusion This method is simple,accurate,sensitive and applicable for determination of the content and entrapment efficiency of itraconazole transfersomes.

关 键 词：伊曲康唑 传递体 包封率 色谱法 高效液相 

分 类 号：R927.2[医药卫生—药学]