HPLC法同时测定人血浆中帕罗西汀和喹硫平浓度  

Determination of Human Plasma Concentration of Paroxetine and Quetiapine by HPLC

作　　者：张岳春[1] 凌卫明[1] 张玉琦[1] 

机构地区：[1]南京医科大学附属无锡市精神卫生中心,江苏无锡214151

出　　处：《标记免疫分析与临床》2012年第5期293-296,共4页Labeled Immunoassays and Clinical Medicine

摘　　要：目的建立同时测定人血浆中帕罗西汀和喹硫平浓度的高效液相色谱(HPLC)法。方法采用HPLC法进行测定:Inertsil ODS-C18柱(4.6×150mm,5μm)为色谱柱,流动相为乙腈-磷酸二氢钠(0.05 mol/L磷酸二氢钠)(39∶61),流速为1.0mL/m in,检测波长为210nm,柱温为40℃,以乙酸乙酯-二氯甲烷(体积比75:25)为萃取剂。结果帕罗西汀在10～640μg/L、喹硫平在20～1200μg/L范围内峰面积与浓度呈良好线性关系,检测限分别为5μg/L,8μg/L。结论该方法简单、快速、灵敏、准确,可用于临床帕罗西汀与喹硫平的血药浓度监测和药动学研究。Objective To establish a method for determining paroxetine and quetiapine in human plasma by HPLC.Methods A reverse phase HPLC system was performed on Inertsil ODS-C18 column（4.6×150 mm,5μm） with the mobile phase consisted of acetonitrile-0.05 mol/L NaH2PO4（39：61）.The flow rate was 1.0 mL/min and the detection wavelength was at 210 nm.The column temperature was kept at 40℃.Ethylacetate and dichloromethane（75∶25） was used as extracting solvent.Results The calibration curves were linear in the range of 10~640μg/L for paroxetine,20~1200μg/L for quetiapine respectively.The minimum detectable concentration for paroxetine and quetiapine were 5μg/L and 8μg/L respectively.Conclusion The method is simple,quick,sensitive and accurate,which can be used for clinical drug monitoring and pharmacokinetics studies of paroxetine and quetiapine.

关 键 词：帕罗西汀 喹硫平 血药浓度 高效液相色谱法 

分 类 号：R969[医药卫生—药理学]