阿托伐他汀在1-磷酸鞘氨醇诱导心肌细胞肥大中的作用  

Effects of atorvastatin on cardiomyocyte hypertrophy induced by sphingosine-1-phosphate

作　　者：熊升林[1,2] 刘行[1] 莫中成[1] 易光辉[1] 

机构地区：[1]南华大学心血管病研究所,衡阳421000 [2]湖南省攸县人民医院

出　　处：《中国心血管杂志》2012年第5期372-375,共4页Chinese Journal of Cardiovascular Medicine

基　　金：国家自然科学基金项目(81100211);湖南省教育厅重点项目(09A078)~~

摘　　要：目的研究阿托伐他汀对1-磷酸鞘氨醇(S1P)诱导乳鼠心肌细胞肥大反应中的作用。方法原代培养乳鼠心肌细胞,测定心肌细胞体积和[3H]-亮氨酸掺入量作为心肌细胞肥大的指标。乳鼠心肌细胞使用不同浓度的阿托伐他汀(atorvastatin),加入S1P,[3H]-亮氨酸掺入量作为心肌细胞蛋白摄取量;分别用qPCR和Western blot法检测心肌细胞的β-肌球蛋白(β-MHC)的mRNA和蛋白质表达水平;用qPCR检测心肌细胞的心房利钠肽(ANF)的mRNA表达水平。结果与S1P组比较,阿托伐他汀10μmol/L处理组[3H]-亮氨酸掺入率减少[(234.89%±31.23%)比(342.23%±31.60%),P=0.205],β-MHC的mRNA和蛋白表达水平下降[(0.59±0.14)比(0.84±0.20),P=0.318]和[(0.55±0.09)比(0.98±0.15),P=0.223],ANF的mRNA表达水平降低[(0.51±0.13)比(0.76±0.19),P=0.445];与S1P组比较,阿托伐他汀20μmol/L处理组[3H]-亮氨酸掺入率明显减少[(189.07%±17.69%)比(342.23%±31.60%),P<0.01],β-MHC的mRNA和蛋白表达水平显著下降[(0.50±0.12)比(0.84±0.20),P<0.01]和[(0.35±0.08)比(0.98±0.15),P<0.01],ANF的mRNA水平明显降低[(0.47±0.12)比(0.76±0.19),P<0.01]。结论阿托伐他汀可抑制S1P诱导的心肌细胞肥大,并可减少S1P诱导的β-MHC和ANF表达。Objective To investigate the effects of atorvastatin on cardiomyocyte hypertrophy induced by Sphingosine-1-phosphate（S1P）. Methods Hypertrophic neonatal rat cardiomyocytes model was made by S1P stimulation.Then the heart cells were treated with different concentration of atorvastatin.Protein compound rate of neonatal rat cardiomyocytes was measured by -Leucine incorporation method.The expression of β-MHC mRNA and protein was detected by qPCR and Western blot.The expression of ANF mRNA was detected by qPCR. Results Compared to control group,-leucine incorporation was decreased [（234.89%±31.23%） vs.（342.23%±31.60%）,P=0.205],β-MHC mRNA and protein levels were reduced [（0.59±0.14） vs.（0.84±0.20）,P=0.318 and（0.55±0.09） vs.（0.98±0.15）,P=0.223] and ANF mRNA expression was decreased [（0.51±0.13） vs.（0.76±0.19）,P=0.445] in atorvastatin 10 μmol/L group.Compared to control group,-leucine incorporation was significantly decreased [（189.07%±17.69%） vs.（342.23%±31.60%）,P0.01],β-MHC mRNA and protein levels significantly reduced [（0.50±0.12） vs.（0.84±0.20） and（0.35±0.08） vs.（0.98±0.15）,both P0.01] and ANF mRNA expression significantly decreased [（0.47±0.12） vs.（0.76±0.19）,P0.01] in atorvastatin 20 μmol/ L group. Conclusions Atorvastatin can inhibit neonatal rat cardiomyocytes hypertrophy induced by S1P as well as the expressions of β-MHC and ANF mRNA.

关 键 词：阿托伐他汀 1-磷酸鞘氨醇 心肌细胞肥大 心房利钠肽 

分 类 号：R541[医药卫生—心血管疾病]