脑室出血后的大鼠脑干组织中去甲肾上腺素含量的变化  

Brainstem norepinephrine adrenaline level changes rats

作　　者：吴勇[1] 谭华[1] 李小刚[1] 

机构地区：[1]四川省泸州医学院附属医院神经内科,646000

出　　处：《神经疾病与精神卫生》2012年第5期475-477,共3页Journal of Neuroscience and Mental Health

摘　　要：目的通过观察大鼠脑室出血后脑组织中去甲肾上腺素（NE）含量，探讨其在脑室出血中的损伤作用，对诊断疾病、预测病情及预后、指导治疗有着实际的临床意义。方法将60只大鼠随机分为正常组、假手术组、脑室出血组（IVH组）。IVH组取自体动脉血方法制备大鼠原发性脑室出血（PIVH）模型，假手术组大鼠只切开头皮和颅骨钻孔，不做自体动脉血注射。分别在术后5个时间点取脑干组织采用ELISA法检测其含量。结果IVH组去甲肾上腺素舍量在6h、1d、3d与正常组、假手术组比较差异有统计学意义（P〈0．05），从6h开始逐渐升高，1d达最高峰，在注血3d后有下降趋势，但仍高于正常组、假手术组。结论IVH后，大鼠脑干组织去甲肾上腺素含量增加，因此去甲肾上腺素在脑室出血后脑组织损伤中可能起着重要作用，并对外周组织产生继发性损害，导致病情加重。Objective To investigate the changes of noradrenaline NE/NA in brain tissue follow- ing ventricular hemorrhage in rats in order to understand the role of NE/NA in brain damage resulted from ventricular hemorrhage. It provides helpful information to clinical practice for diagnoses and treat- ment of the disease and prediction of the disease outcome clinical significance. Methods A total of 60 ex- perimental animals were divided randomly into normal group,sham operation group and the IVH group. The IVH model was produced using the method of injecting their own blood in their brains. Animals in shame operation group received operation of opening the scalp and drilling on the skull. Results The lev- el of noradrenalin of the brainstem： the level of noradrenalin in the IVH group began to rise gradually af- ter 6 hrs and reached the highest point after 1 day, it began to decrease 3 days after injecting blood, but it was still higher than the control group; the differences at 6 hrs, 1 day, and 3 days from that in control group were statistically significant （P〈0.05）. Conclusions After the IVH, the brain tissue nordrenalin level was increased. Thus NE might have important effect on brain tissue damage after the ventricular hemorrhage, cause other organs secondary lesion, and aggravate illness.

关 键 词：脑室出血 去甲肾上腺素 

分 类 号：R743.34[医药卫生—神经病学与精神病学]