瘦素预处理对小鼠心肌冷/热缺血再灌注损伤的差异性  

Differences research of leptin pretreatment on cold/hot myocardial ischemia reperfusion injury in mice

作　　者：吕祥威[1] 徐彤彤[1] 武琦[1] 覃泱[1] 

机构地区：[1]桂林医学院附属医院特需病区,广西桂林541001

出　　处：《中国老年学杂志》2012年第20期4436-4437,共2页Chinese Journal of Gerontology

基　　金：广西省科技厅广西科学研究与技术开发计划项目课题(桂科攻1140003B-76);广西医疗卫生重点科研课题(重2010050);广西研究生教育创新计划项目(桂学位〔2011〕35号-558)

摘　　要：目的研究同种小鼠腹部心脏移植模型中,瘦素预处理对心肌冷/热缺血再灌注损伤(IRI)的差异性。方法 30只雄性C57BL/6小鼠被随机分为3组:(1)心肌冷IRI组:瘦素预处理(术前30 min予腹腔注射重组瘦素,剂量为50μg/kg。)后行同种间小鼠腹部心脏移植,供体置于4℃UW液中保存6 h;(2)心肌热IRI组;(3)心肌缺血再灌注模型对照组(MIRI组)。分别于术后6 h收获标本,观察组织形态学变化,检测小鼠移植心脏及血清中性粒细胞活性,炎性细胞因子水平等。结果心肌冷/热IRI两组对照比较,小鼠血清心肌肌钙蛋白(TnI)、心肌髓过氧化物酶(MPO)活性、血清细胞炎性因子肿瘤坏死因子(TNF)-α,白介素(IL)-1β,IL-6水平无明显差异(P>0.05)。结论瘦素预处理虽然可以减轻心肌冷/热IRI,减轻炎症反应,但是小鼠心肌冷/热IRI之间的差异性无统计学意义。Objective To detect the differences of the leptin pretreatment on cold/hot myocardial ischemia reperfusion injury （MI- RI） in a routine cardiac transplant model. Methods Syngeneic heart transplants were performed in 30 male C57BL/6 mice and were ran- domized into 3 groups： ①cardiac transplant group; ②leptin pretreatment group, leptin 50 μg/kg in 200 μ1 NS injected intraperitoneally be- fore transplantation （ at about 30 min before reperfusion） ; ③MIRI group. The grafts were retrieved at 6 h after reperfusion. Grafts and seium samples were collected for leptin measurement, cytokines analysis and histological analysis to assess intra-graft inflammation. Results The serum troponin I, tumor necrosis factor-alpha, interleukin-1 β and interleukin-6 levels and myocardial myeloperoxidase level are not obvious- ly differences （P 〉 0. 05 ）. Conclusions Pretreatment of leptin is a feasible and effective method of attenuating ischemia/reperfusion injury and inflammatory reaction, protecting cardiac function. The differences of leptin pretreatment on cold/hot MIRI in mice are not statistically significant.

关 键 词：瘦素预处理 心肌冷/热缺血再灌注损伤 心脏移植 炎性细胞因子 

分 类 号：R541.4[医药卫生—心血管疾病]