香石竹环斑病毒多种PCR检测方法的建立与评价  

Development of a Few of PCR Methods for Detecting Carnation Ringspot Virus

作　　者：崔学慧[1] 陈舜胜[1] 于子翔[2] 于翠[2] 杨翠云[2] 

机构地区：[1]上海海洋大学食品学院,上海201306 [2]上海出入境检验检疫局,上海200135

出　　处：《上海交通大学学报（农业科学版）》2012年第5期58-63,共6页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：上海市科委技术标准专项(10DZ0503300;11dz0502700)

摘　　要：以香石竹环斑病毒(Carnation ringspot virus,CRSV)的5个分离物为研究对象,分别建立了快速检测香石竹环斑病毒的多种PCR方法。结果表明,这些方法的灵敏度都比DAS-ELISA的灵敏度高,其中SYBR Green RT-PCR的灵敏度最高,可检测CRSV RNA的最低量是6.4pg。免疫磁珠RT-PCR(IMS-RT-PCR)和普通RT-PCR最低可从400ng的带毒叶片中检出CRSV,而免疫捕获RT-PCR(IC-RT-PCR)最低灵敏度可从40ng的带毒叶片中检出CRSV,是普通RT-PCR的10倍。A few of PCR methods were established for the detecting 5 isolated strains of Carnation ringspot virus （CRSV）. The results showed that the sensitivities of all the PCR methods were higher than those of DAS-ELISA,and among these methods, the SYBR Green RT-PCR was most sensitive with a minimum detectable limit of 6.4 pg RNA of CRSV in sample. The minimum detectable quantity of sample for CRSV detection needed by IMS-RT-PCR and RT-PCR were 400 ng,but IC-RT-PCR only needed 40 ng of sample and the minimum sensitivity of IC-RT-PCR is the normal RT-PCR of 10 times.

关 键 词：香石竹环斑病毒 RT—PCR 免疫捕获RT—PCR免疫磁珠RT—PCR SYBR Green RT—PCR 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]