雌雄鸡胚性腺microRNA表达检测及靶基因预测分析  

Analysis on the Expression and Target Genes of miRNAs in Male and Female Gonads of Chicken Embryos

作　　者：何川[1] 赵乐乐[1] 翟正晓[1] 高宏巍[1] 蒲弘俊[1] 陈永灿[1] 汤琳琳[2] 杨凯旋[2] 李世玉[2] 黄启忠[2] 孟和[1] 

机构地区：[1]上海交通大学农业与生物学院,上海200240 [2]上海市农业科学院畜牧兽医研究所,上海201106

出　　处：《上海交通大学学报（农业科学版）》2012年第5期72-77,共6页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：上海市自然科学基金项目(08ZR1410800);上海市科技兴农重点攻关项目[沪农科攻字(2009)第2-1号];国家肉鸡产业体系项目(CARS-42-Z05)

摘　　要：动物胚胎发育时期伴随着复杂的基因表达和调控过程,特别是microRNA的基因转录后调控。实验应用二代测序技术测定了发育至6.5d雌雄鸡胚性腺中miRNA表达情况,并对差异表达miRNA进行了靶基因分析。检测分析结果显示,在雄性样品中发现375种miRNA,雌性样品中发现372种miRNA。针对30种在两性性腺中显著差异表达的miRNA,实验采用3个软件(Targetscan 6.0,MirTarget2与miRanda)进行靶基因预测分析,共预测出12 477个靶基因,这些基因显著富集在205个GO分类和11条信号通路中。实验为鸟类性别分化调控机制研究和miRNA靶基因分析提供了基本实验数据和方法学参考。The process of animal embryonic development is associated with complicated gene regulation, especially post-transcriptional regulation of microRNAs. In this work, with next-generation sequencing, the expression of miRNAs in gonads of 6. 5 d male and female chicken embryos was evaluated. The results showed that 375 miRNAs were found in male sample,while 372 miRNAs were found in female sample. We predicted 12 477 comdidate target genes for 30 significantly differentially expressed miRNAs with three software tools （TargetScan 6.0,MirTarget2 and miRanda）. Further enrichment analysis found these genes significantly focused on 205 GO terms and 11 pathways. This investigation provided basic experimental data for the regulation mechanism of avian gender and methodological references for miRNA target genes analysis.

关 键 词：鸡胚 性腺 微小RNA 靶基因预测 第2代测序技术 

分 类 号：S831[农业科学—畜牧学]