苹果酸度基因(Ma)SSR标记及遗传分析  

Genetic Analysis and SSR Markers of Acid Gene Ma in Apple

作　　者：王雷存[1] 樊红科 高华[1] 赵政阳[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100 [2]陕西农村科技开发中心,西安710054

出　　处：《园艺学报》2012年第10期1885-1892,共8页Acta Horticulturae Sinica

基　　金：国家苹果产业技术体系项目(nycytx-08-01-03);陕西省科技攻关项目(2010K01-04-1);陕西省‘13115’重大专项资助项目(2010ZDKG-69)

摘　　要：研究果实含酸量的遗传特性及其酸度基因的分子标记可以为果品品质育种提供辅助手段。以果实低酸的'短枝富士'(Spur Fuji)和高酸的'粉红女士'(Pink Lady)F1代群体(216株)为试材,利用SSR(simple sequence repeat)技术,结合集群分类分析法(bulked segregation analysis,BSA)进行了苹果酸度基因(Ma)分子标记研究。经过102对SSR引物的筛选,获得了与果实酸性状紧密连锁的分子标记CH03d12104和CH03d12118两个位点,连锁距离分别为3.24和2.31cM。分析表明Ma1与Ma2对果实含酸量有控制作用,Ma对ma表现为完全显性。Researches on heredity characters of acid contents and molecular markers of genes controlling acidness can provide supplementary means for apple breeding in quality.A DNA marker linked to the acid gene（Ma） in apple was explored based on the population of 216 progenies of‘Spur Fuji’× ‘Pink Lady’by simple sequence repea（tSSR）technique combined with bulk segregation analysi（sBSA）. One hundred and two primer pairs were screened,and two SSR markers（CH03d12 104 ＋ CH03d12 118 ） closely linked to Ma gene was identified. The linkage distance of two markers locus is 3.24 and 2.31 cM, respectively. The SSR marker analysis showed that the fruit acid trait was governed by Ma 1 and Ma 2 ,and Ma was completely dominant to ma.

关 键 词：苹果 Ma基因 SSR标记 遗传分析 

分 类 号：S661.1[农业科学—果树学]