银杏类黄酮糖基转移酶基因全长序列克隆及表达分析  

Full Length cDNA Cloning and Expression Analysis of UFGT Gene from Ginkgo biloba

作　　者：张传丽[1,2] 陈鹏[1] 仲月明[1] 周长远[1] 沈丹红[1] 蒋菲[1] 

机构地区：[1]扬州大学园艺与植物保护学院,江苏扬州225009 [2]扬州大学生物科学与技术学院,江苏扬州225009

出　　处：《园艺学报》2012年第10期1903-1912,共10页Acta Horticulturae Sinica

基　　金：国家农业综合开发项目(GH32311002);江苏省林业三项工程项目(lxsx[2011]16);江苏省研究生科研创新计划项目(CXZZ11_0975)

摘　　要：以银杏(Ginkgo biloba L.)雄株叶片为试材,采用同源基因克隆及RACE-PCR方法,克隆到了类黄酮糖基转移酶(UDP-glycose:flavonoid glycosyltransferase,UFGT)基因GbUFGT的全长cDNA序列,其在GenBank中的注册号为JN640564.2。该基因编码区长1491bp,编码496个氨基酸。GbUFGT蛋白具有保守的PSPG基序、UDP-葡萄糖基转移酶和UDP-葡萄糖醛酸基转移酶结构域,与其他植物中的UFGT蛋白同源性较高。GbUFGT基因组序列与其cDNA序列相同,无内含子。半定量RT-PCR结果表明,GbUFGT在整个银杏叶片发育期均可较高水平的表达,且不同发育期表达水平无明显变化,属非发育时期特异性基因。A full length cDNA of UDP-glycose：flavonoid glycosyltransferase gene was obtained from ginkgo leaf for the first time,using degenerate primer PCR and RACE protocol. The sequence which was designated as GbUFGT,had been embodied in GenBank database with an accession No. of JN640564.2. The cDNA sequence and genomic DNA sequence of GbUFGT were the same,in other words,GbUFGT was an intron-less gene. The coding region of GbUFGT was 1 491 bp long,encoding 496 amino acids. The deduced GbUFGT protein containing a typical conversed plant secondary product glycosyltransferase （PSPG）motif,and an UDP-glucoronosyltransferase and UDP-glucosyltransferase domain,showed high identities to other plant UFGTs. Semi-quantitative RT-PCR analysis revealed that GbUFGT expressed highly at the whole developmental stages of ginkgo leaf,and the expression levels were similar in different developmental periods,displaying developmental-stage independence.

关 键 词：银杏 类黄酮糖基转移酶 CDNA 基因组 表达模式 

分 类 号：S664.3[农业科学—果树学]