番茄脱水素基因SlDHN2b的克隆与表达分析  

Cloning and Characterization of Dehydrins Gene SlDHN2b in Tomato

作　　者：郭鹏[1] 张士刚[2] 金华[1] 邹吉祥[1] 董燕[3] 姜国斌[1] 

机构地区：[1]大连民族学院环境与资源学院生物技术系,辽宁大连116600 [2]山东泰安市农业局,山东泰安271018 [3]辽宁林业职业技术学院,沈阳110101

出　　处：《园艺学报》2012年第10期2015-2022,共8页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31100489);辽宁省重点农业攻关计划项目(2008207001);中央高校基本科研业务费资助项目(DC12010204)

摘　　要：利用RT-PCR从番茄叶片中获得脱水素基因(dehydrins)的cDNA序列,命名为SlDHN2b。该基因开放阅读框1140bp,编码380个氨基酸。蛋白序列分析表明该蛋白高度亲水。Real-timePCR分析表明该基因受盐、脱水、ABA诱导表达。通过染色体步移技术获得SlDHN2b基因的启动子,PLACE数据库分析预测SlDHN2b启动子序列中含有多种逆境相关的作用元件。Northern杂交表明该基因在叶片中表达量最高,果实、花、茎中次之,根中最少。In present study,a cDNA clone,designated SlDHN2b,was isolated from tomato by RT-PCR. The ORF of SlDHN2b was 1 140 bp and contained 380 amino acid residues. Protein sequence analysis showed that the SlDHN2b was high hydrophilic. Real-time PCR analysis indicated that mRNA accumulation of SlDHN2b was induced by salt stress,abscisic acid（ABA）and dehydration. Analysis of the promoter of PdEPF1 revealed the presence of stress-responsive elements by genome walking kit. Tissue-specific expression indicated that SlDHN2b was mainly expressed in leaves,weakly expressed in bud,flower,stem and the lowest in roots. Analysis of the promoter of SlDHN2b revealed the presence of stress-responsive elements.

关 键 词：番茄 SlDHN2b 克隆 逆境 启动子 

分 类 号：S641.2[农业科学—蔬菜学]