TCRP1在口腔鳞癌中介导顺铂耐药的体内实验研究  

Effects of TCRP1 on mediating resistance to cisplatin in vivo in OSCC

作　　者：谷依学[1,2] 范莎莎[2] 王成昆[2] 彭波[2] 张为家[2] 贺智敏[1,2] 

机构地区：[1]广州医学院附属肿瘤医院肿瘤研究所,广东广州510182 [2]中南大学湘雅医学院肿瘤研究所,湖南长沙410057

出　　处：《中国药理学通报》2012年第11期1574-1578,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 30873088);教育部博士点基金资助项目(No 200805330009)

摘　　要：目的 TCRP1是新近从舌癌耐药细胞系Tca8113/PYM中克隆出来的一个新基因,前期体外实验研究表明TCRP1在口腔鳞癌细胞中能特异地介导对顺铂化疗耐受,本研究将进一步探讨TCRP1在体内实验中介导化疗耐受的作用。方法通过建立TCRP1过/沉默表达裸鼠移植瘤模型,观测TCRP1对化疗药物5-Fu、cDDP的作用敏感性的影响并初步探讨其机制。结果肿瘤生长曲线及肿瘤瘤重结果显示TCRP1能增强裸鼠移植瘤对顺铂的耐药性,而对5-Fu则没有影响。TUNEL检测发现TCRP1高表达的组织中凋亡细胞计数更少。结论 TCRP1是一个新的耐药相关基因,其作用机制与通过增强口腔鳞癌细胞凋亡抗性有关。Aim TCRP1 is a novel gene which was cloned from a multi-drug resistant cell line Tca8113/PYM in previous research.The preliminary functional investigation in vitro indicated that TCRP1 was a new chemo-resistance related gene,which mediated specifically resistance to cisplatin in Oral Squamous Cell Carcinoma（OSCC） cells.This study aims to futher explore the effect of TCRP1 in OSCC in vivo.Methods The effect of TCRP1on sensitivity to cisplatin and 5-Fu was observed through establishing the ectopic tumor animal model of over/silence expression of TCRP1,and then the initiatory mechanism was imvestigated.Results The outcome of tumor growth curves and tumor weight displayed that TCRP1 significantly increased the resistance to cisplatin in OSCC ectopic tumor,but not to 5-Fu.And the TUNEL assay indicated there were fewer number of apoptosis cells in up-regulated TCRP1 tumor tissue.Conclusion TCRP1 is a novel chemo-resistance related gene,and its functional mechanism is correlated with reducing the cells apoptosis induced by cisplatin.

关 键 词：肿瘤 口腔鳞癌 顺铂 耐药 TCRP1 体内实验 凋亡 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学] R73-34[医药卫生—基础医学]