应用抗多肽抗体鉴别新城疫病毒强弱毒株  

Preparation of Newcastle Disease Virus (NDV)antipeptide Antibody for Analysis of Virulent and Avirulent NDV Strains

作　　者：古长庆[1] 金宁一[1] 金扩世[1] 郭志儒[1] 龚伟[1] 王兴龙[1] 李萍[1] 刘子 殷震[1] 

机构地区：[1]解放军军需大学军事兽医研究所,吉林长春130062

出　　处：《中国兽医学报》2000年第2期121-123,共3页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目! ( 3 9893 2 90 -4 -3 ) ;国家"863"课题! ( 10 1-0 5 -0 3 -1)

摘　　要：:在克隆我国流行的新城疫病毒 ( NDV)强毒株 F4 8E8株、四平株及弱毒株长春株、V4株的 HN和 F基因并进行测序的基础上 ,针对我国流行的 NDV强毒株 F蛋白前体 ( F0 )的 F2片段的特异结构 ,人工合成特异性多肽 ,将其与小牛血清白蛋白 ( BSA)化学偶联制备成全抗原 ,免疫小鼠制备出抗多肽血清。经 ELISA检测 ,该抗体与 NDV强毒株呈强阳性反应 ,而与鸡痘病毒、鸡传染性法氏囊病病毒、NDV弱毒株长春株和 V4株呈阴性反应。试验结果表明 ,该抗体可以用于After the sequences of F gene and HN gene of the Chinese NDV strains (F48E8, Siping, Changchun and V4) were amplified and sequenced,peptide was synthesized that correspond to cleaved and trimmed carboxyl termini of the F2 polypeptide regions of fusion(F) protein precursor (F0 protein) in Chinese virulent NDV strains. The purified peptide was conjugated to bovine serum albumin using glytaraldehyde.Polyclonal mice antibody against the peptide reacted with intact egg propagated virions of virulent NDV strains in an ELISA, but didn′t react with that of avirulent NDV strains, fowlpox virus and infectious bursal disease virus. The result showed that the antipeptide antibody can be applied in the rapid analysis between the virulent and the avirulent NDV strains. [WT5HZ]

关 键 词：新城疫病毒 抗多肽抗体 毒力鉴定 诊断 

分 类 号：S585.315.3[农业科学—作物学]