正交分析法优选半乳糖化羧甲基壳聚糖磁性纳米载体的制备条件  

Optimize the preparing condition of galactosylated-carboxymethyl chitosan-magnetic iron oxide nanoparticles by orthogonal method

作　　者：薛万江[1] 冯盈[1,2] 李鹏[1] 郭益冰[2] 俞铖[1] 毛勤生[1] 

机构地区：[1]南通大学附属医院普外科,南通226001 [2]南通大学附属医院外科综合实验室,南通226001

出　　处：《南通大学学报（医学版）》2012年第5期350-352,共3页Journal of Nantong University(Medical sciences)

基　　金：国家自然科学基金青年基金资助项目(81000984);江苏省高校自然科学研究面上资助项目(10KJB320014);南通市社会发展科技计划资助项目(S2010019);江苏省"六大人才高峰"资助项目(2011-WS066)

摘　　要：目的:优选半乳糖化羧甲基壳聚糖磁性纳米载体(galactosylated-carboxymethyl chitosan-magnetic iron ox-ide nanoparticles,Gal-CMCS-Fe3O4NP)的制备条件。方法 :采用正交分析试验方法 ,以Fe3O4NP与羧甲基壳聚糖(car-boxymethyl chitosan,CMCS)质量比、pH值和搅拌时间为考察因素,纳米载体的粒径和磁响应性为考察指标。结果 :Fe3O4NP与CMCS的质量比为1∶2、pH值为7、搅拌时间为1 h时为最佳制备条件,最终所制备的Gal-CMCS-Fe3O4NP的平均粒径为20 nm,具有较好的磁响应性。结论:获得较满意的Gal-CMCS-Fe3O4NP的制备条件,纳米载体性状符合要求。Objective： To optimize the preparing condition of galactosylated-carboxymethyl chitosan-magnetic iron oxide nanoparticles（Gal-CMCS-Fe3O4NP）.Methods： Orthogonal experiment was designed with the particle diameter and magnetic property as index,and with quality ratio between Fe3O4NP and carboxymethyl chitosan（CMCS）,pH and the stirring time as factors.Results： The orthogonal analysis indicated that the optimal preparation conditions was followed,quality ratio between Fe3O4NP and carboxymethyl chitosan（CMCS） was 1∶2,pH was 7 and the stirring time was 1 hour.The final Gal-CMCS-Fe3O4NP have good magnetic property,and the average particle diameter is 20 nm.Conclusion： With this method,a satisfactory preparing condition can be obtained,and the character of the nanoparticles is desirable.

关 键 词：正交试验 磁性纳米粒子 羧甲基壳聚糖 半乳糖 

分 类 号：Q782[生物学—分子生物学]