角质细胞生长因子促进大鼠胰腺导管上皮细胞体外增殖  

Keratinocyte growth factor promotes proliferation of rat pancreatic ductal epithelium cells in vitro

作　　者：吴刚[1] 张晓健[1] 白光[1] 

机构地区：[1]辽宁医学院附属第一医院肝胆外科,辽宁锦州121000

出　　处：《基础医学与临床》2012年第11期1279-1283,共5页Basic and Clinical Medicine

基　　金：辽宁省教育厅基金项目(L2010290)

摘　　要：目的探讨不同浓度的角质细胞生长因子(KGF)对大鼠胰腺导管上皮细胞增殖的影响,寻求最佳浓度。方法免疫细胞化学染色及RT-PCR方法鉴定SD大鼠胰腺导管上皮细胞;不同浓度的KGF刺激胰腺导管上皮细胞增殖,寻求最佳浓度。结果大鼠胰腺导管上皮细胞表达Nestin和CK19,不表达Insulin及Glucagon;不同浓度KGF刺激胰腺导管上皮细胞2 d后,细胞均有不同程度增殖,20μg/L KGF作用2 d时,细胞数为0.35±0.03,显著高于对照组的0.27±0.02(P<0.01)。结论 KGF在本实验的剂量范围内可促进胰腺导管上皮细胞增殖,20μg/L为促进增殖的最佳浓度。Objective To investigate the effect of keratinocyte growth factor（KGF） at different concentrations on the proliferation of pancreatic ductal epithelial cells（PDECs）.Methods The PDECs were identified by immunocytochemical stain and RT-PCR.PDECs counting was carried out at different KGF concentrations.Results Nestin and CK19 were positively expressed in PDECs by RT-PCR and immunocytochemical stain;PDECs were stimulated by different concentrations of KGF after 2 days,and PDECs number stimulated by 20 μg/L KGF was 0.35±0.03,much higher than that of the control group which was 0.27±0.02.Conclusions KGF at different concentrations can significantly stimulate the proliferation of PDECs,the optimized concentration found in our tests is 20 μg/L.

关 键 词：胰腺导管上皮细胞 角质细胞生长因子 增殖 糖尿病 

分 类 号：R587.1[医药卫生—内分泌]