按摩抑制兔钝挫伤骨骼肌瘢痕形成的机制研究  

How massage inhibits scar tissue formation in skeletal muscles after blunt injury

作　　者：刘仁建[1] 唐成林[1] 邹敏[1] 郜婕[1] 谢辉[1] 陈晓琳[1] 

机构地区：[1]重庆医科大学中医药学院中医药研究室,重庆400016

出　　处：《中华物理医学与康复杂志》2012年第10期742-746,共5页Chinese Journal of Physical Medicine and Rehabilitation

摘　　要：目的观察按摩对实验兔受损股四头肌转移生长因子（TGF—β1）及Ⅰ型胶原（COL—Ⅰ）mRNA表达的影响，以探讨按摩抑制瘢痕形成的相关机制。方法共选取健康成年雄性新西兰大白兔40只，采用随机数字表法将其分为正常对照组（4只）、自然恢复组（20只）及按摩组（16只）。正常对照组饲养期间未给予特殊处理，采用自制打击器将自然恢复组及按摩组实验兔制成兔右后肢股四头肌损伤模型。按摩组实验兔于制模后第5天时给予按摩治疗，自然恢复组制模后未给予按摩治疗。于制模后7d、11d、15d及19d时采用实时定量RT—PCR法检测各组实验兔TGF一β1、COL—ⅠmRNA表达情况。结果在制模后第7天时，发现按摩组TGF—β1、COL—ⅠmRNA表达量与自然恢复组间差异无统计学意义（P〉0．05），在制模后第11，15及19天时，发现按摩组TGF一β1、COL—ⅠmRNA表达量均显著低于自然恢复组水平（P〈0．05）。结论按摩能显著降低实验兔受损股四头肌TGF一β1．及COL—ⅠmRNA表达，有助于抑制瘢痕过度形成，从而促进受损肌组织修复。Objective To investigate the effect of massage on quadriceps femoris repair after injury by ex ternal force and the expression of transforming growth factor β1 （TGF-β1 ） and collagen-I （COL-Ⅰ） mRNA. To explore the molecular mechanisms inhibiting scar tissue formation and promoting muscle repair. Methods Forty New Zeal and white rabbits weighing （2.0 ± 0.5 ） kg were randomly divided into a normal control group （A） （ n = 4） , a self- repair group （B） （n =20, further divided into the 3rd, 7th, llth, 15th and 19th day time points） , and a massage group （C） （n = 16, further divided as in group B）. In group A the rabbits were not treated, as normal controls. In groups B and C rabbit models of quadriceps femoris injury were prepared using a self-made beater. In group B no massage therapy was given as a natural recovery control; in group C, massage therapy was given after 5 days. Real time quantitative PCRs were used to detect TGF-β1 and COL-Ⅰ mRNA expression. Results There was no significant difference between groups B and C in the expression of TGF-β1 or COL-Ⅰ mRNA on day 7. At the later time points, expression of both mRNAs in group C was significantly less than in group B. Conclusion Massage can effectively reduce the expression of TGF-β1I and COL-Ⅰ mRNA, inhibit excessive sear formation and promote repair of injured tissue.

关 键 词：按摩 骨骼肌 转移生长因子Β1 Ⅰ型胶原 

分 类 号：R873[医药卫生—运动医学]