C5aR1促进神经干细胞增殖作用的实验研究  

The role of C5aR1 in promoting the proliferation of neural stem cells

作　　者：燕增奎[1] 秦茂林[1] 刘运来[1] 李成仁[1] 刘建军[1] 肖岚[1] 

机构地区：[1]第三军医大学基础部组胚教研室,重庆400038

出　　处：《免疫学杂志》2012年第11期946-949,共4页Immunological Journal

基　　金：国家自然基金项目(31171046);重庆市自然科学基金项目(2011BB5036)

摘　　要：目的研究C5aR1在新生小鼠脑内及体外培养神经干细胞内的表达规律,探索C5aR1对神经干细胞增殖调节的作用。方法以免疫组化方法检测在新生小鼠脑内及体外培养的神经干细胞内C5aR1的表达;培养C5aR基因敲除小鼠来源的神经干细胞,同时利用C5aR拮抗剂处理正常小鼠来源的神经干细胞,分别观察神经干细胞的增殖状态,分析C5aR1对神经干细胞增殖的调节作用。结果 1)在新生小鼠脑内,C5aR1主要表达于海马和室下区等神经干细胞分布区域;2)C5aR1与nestin共表达于体外培养的神经干细胞内;3)C5aR特异性拮抗肽下调体外培养的神经干细胞内nestin表达并抑制其增殖;C5aR1基因敲除,神经干细胞的成球能力明显降低。结论 C5aR1在脑内以及神经干细胞内均有表达,对神经干细胞的增殖具有促进作用,其具体机制还有待进一步的实验研究。This study aimed to discover the role of C5aR1 in the proliferation of neural stem cells（NSCs） derived from mouse brain.Firstly,ICC was performed to investigate the expression of C5aR1 in the postnatal mouse brain,and found that the most of C5aR1 was expressed in neonatal mouse brain including hippocampus and lateral ventricle where there were affluent NSCs.Meanwhile,we found there was high level expression of C5aR1 in NSCs in vitro.And we also found C5aR1 was co-expressed with nestin,a marker of NSCs.These results suggested that C5aR1 involved in the proliferation of NSCs.Furthermore,the effect of C5aR1 on proliferation of NSCs was confirmed with C5aR-a,a C5aR antagonist,and C5aR1-KO mouse model.MMT test displayed the proliferation of NSCs was decreased after the 10-100 ng/ml C5aR-a treatment,and ICC showed the C5aR-a down-regulated the expression of nestin in vitro.On the other hand,we found the formation of neurospheres was inhibited in NSCs derived from C5aR-KO mouse.All these results suggested that the C5aR1 is required in the proliferation of NSCs.In short,our data showed C5aR1 plays an important role in the proliferation of NSCs.

关 键 词：补体 C5aR1 神经干细胞 增殖 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]