5-羟色胺对大鼠膀胱传入神经活动的影响  

Effect of 5-hydroxytryptamine on activity of bladder afferent nerves in rats

作　　者：孙俏[1] 孙碧英[1] 罗萍[1] 王莹萍[1] 董莉[1] 戎伟芳[1] 

机构地区：[1]上海交通大学基础医学院生理学教研室,上海200025

出　　处：《上海交通大学学报（医学版）》2012年第10期1292-1295,1301,共5页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海市科委自然基金资助(10ZR1417300)~~

摘　　要：目的观察5-羟色胺(5-HT)对膀胱传入神经自发放电活动和机械敏感性的影响。方法采用离体大鼠膀胱—盆神经标本,观察膀胱腔内给予不同浓度5-HT后,盆神经自发放电频率及充胀膀胱引起的放电活动的变化。结果膀胱未充胀时,盆神经仅有少量自发放电活动(<10 imp/s);充胀时,盆神经放电频率随膀胱内压力递增而升高;膀胱腔内给予5-HT(1～30μmol/L,0.1 mL)后,盆神经自发放电频率呈剂量依赖性增加,对0～15 mmHg范围(低压区)充胀刺激的反应增强。结论 5-HT能直接激活膀胱低阈值传入神经,并增强其对机械刺激的反应。Objective To investigate the effect of 5-hydroxytryptamine（5-HT） on the spontaneous and mechanosensitive activity of bladder afferent nerves.Methods The effects of intravesical instillation of different concentrations of 5-HT on the spontaneous and distension-induced pelvic afferent nerve activity were observed in the isolated rat bladder/pelvic nerve preparations.Results At rest,with the bladder empty,there was little background activity（10 imp/s） in the pelvic nerve branch innervating the bladder.During ramp distension,the afferent nerve activity increased progressively as the intravesical pressure rose.Intraluminal instillation of 0.1 mL 5-HT（1 to 30 μmol/L） caused an increase of the ongoing afferent discharge and potentiated the low threshold（0-15 mmHg） mechanosensory responses in a concentration-dependent manner.Conclusion 5-HT directly activates low threshold bladder afferent fibers in the pelvic nerve,and can potentiate their mechanosensitivity.

关 键 词：5-羟色胺受体 膀胱传入神经 机械敏感性 膀胱—盆神经标本 

分 类 号：R338[医药卫生—人体生理学]