寻常性银屑病患者外周血Th17细胞相关因子的表达  

Expressions of the Th17 cells related cytokines in psoriasis vulgaris

作　　者：刘栋[1] 廖非[2] 李雅琳[1] 乔树芳[1] 徐丽敏[1] 焦振山[1] 

机构地区：[1]天津市长征医院皮肤科,天津300021 [2]苏州市第五人民医院皮肤科,江苏苏州215007

出　　处：《临床皮肤科杂志》2012年第10期590-592,共3页Journal of Clinical Dermatology

摘　　要：目的:探讨Th17细胞相关因子白介素(IL)-23、IL-17A、IL-17F、IL-22与银屑病的关系与意义。方法:30例寻常性银屑病患者分为进行期15例,稳定期15例;30例正常人作为对照,取外周血,采用双抗体夹心ELISA法检测血浆IL-23的含量,实时荧光定量逆转录聚合酶链反应(FQ-RT-PCR)技术检测外周血中外周血单个核细胞(PBMC)IL-17A mRNA、IL-17FmRNA、IL-22 mRNA的相对表达量。结果:进行期银屑病患者血浆IL-23浓度、PBMC IL-17A mRNA、IL-17F mRNA、IL-22mRNA的表达均明显高于正常对照组,进行期患者PBMCIL-17A mRNA、IL-22 mRNA的表达高于静止期患者,而稳定期患者上述指标的表达与正常对照无统计学差异。结论:Th17细胞可能参与进行期寻常性银屑病病理过程。Objectives： To detect the expressions of Thl7 cells related cytokines including IL-17A, IL-17F, IL-22 and IL- 23 in peripheral blood of patients with psoriasis vulgaris, and further illustrate the pathogenesis of psoriasis vulgaris. Methods： Peripheral blood was obtained from 15 patients with stable psoriasis vulgaris, 15 patients with progressive psoriasis vulgaris and 30 healthy controls. The level of IL-23 in plasma was determined by ELISA, and the mRNA expression of IL-17A, IL-17F and IL-22 in peripheral blood mononuclear cell （PBMC） detected by FQ-RT-PCR （real time fluorescent quantitative-reverse transcriptase-polymerase chain reaction）. Results： The level of IL-23 in progressive psoriasis was signifi- cantly higher than that of healthy control （P = 0.002）, but this different was not found between the stable psoriasis patients and healthy control （P=0.169）. The levels of IL-17A mRNA, IL-17FmRNA and IL-22 mRNA in progressive psoriasis were significantly higher than those of healthy control （P = 0.007; P = 0.015; P = 0.002）. The levels of IL-17A mRNA and IL-22 mRNA in progressive psoriasis were significantly higher than that in stable psoriasis other than IL-17F mRNA. Conclusions： Thl7 cells may take part in the pathogenesis of progressive psoriasis vulgaris.

关 键 词：银屑病 TH17细胞 白介素-23 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]