流动激活血管内皮细胞中转录因子核因子-кB和激活蛋白-1的表达  

Effect of Different Flow Fields on Nuclear Factor-κB and Activator Protein-1 Expression in Vascular Endothelial Cells

作　　者：杨驾煌[1] 赁可[1] 蒙炜[1] 张尔永[1] 

机构地区：[1]四川大学华西医院心血管外科,成都610041

出　　处：《中国胸心血管外科临床杂志》2012年第5期543-546,共4页Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

基　　金：国家自然科学基金重点资助项目(39830110);国家自然科学基金资助项目(39970734)~~

摘　　要：目的比较血管内皮细胞(endothelial cells,ECs)在不同流场中转录因子核因子-кB(nuclear factor-кB,NF-кB)和激活蛋白-1(activator protein-1,AP-1)的表达规律,为力学信号对基因调控模式的影响提供依据。方法将培养的人脐静脉ECs加载于层流和湍流的离体流室模型中,以作用时程的不同分为6个时间点(0.5 h、1 h、2 h、3 h、4 h和5 h),运用共聚焦显微镜,从蛋白水平观察ECs中NF-кB和AP-1在不同流室中表达的时间规律和空间规律。结果层流中NF-кB的表达高峰出现在1 h段(26.49±1.63,P<0.05),随后转行向下;湍流中NF-кB表达在3 h段升至峰值(34.41±6.43,P<0.05);层流中c-Jun/AP-1表达一过性增高,0.5 h到达峰值(18.95±5.38,P<0.05),随即下降至基线水平;湍流中c-Jun/AP-1表达呈持续缓慢上升趋势(P<0.05)。结论湍流流场对血管ECs中NF-кB和AP-1表达的影响不同于层流,湍流对NF-кB和AP-1的持续激活上调作用可能引起了ECs形态结构和功能行为的病理学改变。Objective To investigate the expression of transcription factors including nuclear factor-κB（NF-κB） and activator protein-1（AP-1） in vascular endothelial cells（ECs） in different flow fields,and provide experimental evidence for mechanical signal effects on gene regulation pattern of ECs.Methods Cultured human umbilical vein ECs were loaded into steady flow chambers of laminar flow or turbulent flow and observed at 6 time points（0.5 h,1 h,2 h,3 h,4 h and 5 h） based on different load time.Spacial and temporal characteristics of NF-κB and AP-1 expression in ECs in different flow chambers were detected at a protein level by laser confocal microscope.Results In laminar flow,NF-κB expression rose to peak at 1 hour（26.49±1.63,P0.05）and then declined.In turbulent flow,NF-κB expression rose to peak at 3 hours（34.41±6.43,P0.05）.In laminar flow,c-Jun/AP-1 expression was transiently elevated,reached its peak at 0.5 hour（18.95±5.38,P0.05）and then fell to its baseline level.In turbulent flow,c-Jun/AP-1 expression rose slowly but steady to peak（P0.05）.Conclusion The effects of turbulent flow on NF-κB and AP-1 expression in ECs are different from those of laminar flow.Up-regulation and activation of NF-κB and AP-1 expression in ECs induced by turbulent flow may cause pathological changes in morphological structure and functional behavior of ECs.

关 键 词：湍流 血管内皮细胞 转录因子核因子-кB 激活蛋白-1 

分 类 号：R543[医药卫生—心血管疾病]