雷公藤内酯醇对Aβ_(1-42)激活的小胶质细胞一氧化氮释放和诱导型一氧化氮合酶表达的影响  

Effects of triptolide on expression of inducible nitric oxide synthase and production of nitrogen monoxide in microglia cells induced by β-amyloid

作　　者：王会玲[1] 周晓春[1] 马春虎[1] 王冰[2] 王晓民[3] 

机构地区：[1]承德医学院生理教研室,河北承德067000 [2]承德医学院附属医院,河北承德067000 [3]首都医科大学,北京100069

出　　处：《首都医科大学学报》2012年第5期643-646,共4页Journal of Capital Medical University

基　　金：河北省2009年医学科学研究重点课题计划(20090581)~~

摘　　要：目的研究雷公藤内酯醇(triptolide,T10)对β-淀粉样蛋白(β-amyloid,Aβ)1-42激活的小胶质细胞一氧化氮(nitrogenmonoxidum,NO)的产生及诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)表达的影响。方法用不同浓度的T10(10-11、10-10、10-9、10-8mol/L)预孵育小胶质细胞12 h,然后加入10μmol/L Aβ1-42共孵育12 h;Griess反应检测上清NO的含量,Western blotting检测iNOS蛋白的表达。结果 Aβ组iNOS的表达和NO释放明显高于对照组(P<0.01)。T10可明显减少Aβ诱导的小胶质细胞iNOS的表达及NO的释放(P<0.05)。结论 T10可抑制Aβ1-42激活的小胶质细胞iNOS蛋白的表达及NO的释放。Objective To investigate the effects of triptolide （T10） on expression of inducible nitric oxide synthase （iNOS） and production of nitrogen monoxidum （NO） in microglia cells induced by β-amyloid （Aβ）1-42. Methods The microglia cells were pre- treated with 10^ - 11^ - 10^ -8 mol/L T10 for 12 h before 10 μmol/L Aβ1-42 were added, and incubated for 12 h together. The production of NO was determined by Griess reaction, and the expression of iNOS was determined by Western blotting. Results Expression of iNOS and production of NO in Aβ group were significantly higher than those in control group（ P 〈 0.01 ）. T10 treatment reduced expression of iNOS and production of NO obviously induced by Aβ from microglia cells （P 〈 0.05）. Conclusion T10 significantly inhibited expression of iNOS and production of NO in microglia cells induced by Aβ. T10 might provide a novel therapy for AD.

关 键 词：阿尔茨海默病 雷公藤内酯醇 小胶质细胞 一氧化氮 诱导型一氧化氮合酶 

分 类 号：R338.2[医药卫生—人体生理学]