室间质评中耐药表型检测结果分析  

Results analysis on external quality assessment of drug-resistant phenotypes in Hunan province

作　　者：何力志[1] 赵艳华[1] 吕岳峰[1] 佘鸥[1] 

机构地区：[1]湖南省临床检验中心,长沙410007

出　　处：《检验医学与临床》2012年第20期2556-2557,共2页Laboratory Medicine and Clinic

摘　　要：目的促进临床微生物实验室积极开展耐药表型检测,提高实验室整体检测水平。方法由临床检验中心统一发放菌种,要求各实验室在给定的几项耐药表型检测中根据鉴定结果选择一至两项,严格按照CLSI推荐的方法进行检测,并在规定的时间内回报结果。结果做耐药表型检测的6株细菌平均鉴定符合率为96%,对它们表型试验的选择以及最终检测结果的影响相对较小。6株菌耐药表型检测平均符合率为71%,其中200710号标本检测符合率较低,MRSA检测符合率为48%,D-试验检测符合率为43%。结论室间质评中增加耐药表型检测能够有效促进临床微生物室重视细菌耐药性检测,提高耐药性检测水平。Objective To promote the clinical microbiology laboratories to carry out the drug-resistant pheno- types detection actively and improve the overall detection level of the laboratory. Methods Strains were uniformly delivered to the laboratories at different levels by the Clinical Laboratory Center. 1 or 2 items of the several given drug-resistant phenotypes tests were chosen by each laboratory according to the identification results, and strictly conducted by the method of CLSI recommending. The test results were sent back within the stipulated time. Results The average correct identification rate of 6 strains of bacteria for conducting drug-resistant phenotypes detection was 96%, which had relatively small impact on the choice of the phenotypic test and final test results. The average coinci- dence rate of 6 strains of bacteria with drug-resistant phenotypes was 71 %. Among them, the detection coincidence rate of No. 200710 specimen was low,which of MRSA was 48%0 ,the detection coincidence rate of D-test was 43%. Conclusion Adding the detection of drug-resistant phenotypes in the external quality assessment activities can effec- tively promote the clinical microbiology to pay close attention to the detection of the bacterial resistance,and improve the level of the drug-resistance detection.

关 键 词：室间质评 耐药性 表型检测 MRSA ESBLS 

分 类 号：R378[医药卫生—病原生物学]