4种方法在检测痰内结核分枝杆菌结果的比较分析  

Comparative analysis on results of 4 different methods for detecting Mycobacterium tuberculosis in sputum

作　　者：吴科明[1] 莫实德[1] 

机构地区：[1]广西壮族自治区防城港市疾病预防控制中心检验科,538021

出　　处：《检验医学与临床》2012年第20期2577-2578,共2页Laboratory Medicine and Clinic

摘　　要：目的对结核病临床诊断方法的应用价值进行比较分析。方法对别采用涂片法、免疫磁珠技术分离涂片法(IMS-涂片)、痰培养法、荧光定量聚合酶链反应(FQ-PCR)对病例组112例和对照组53例痰标本进行检测。结果普通涂片法灵敏度最低为25.00%,其次是IMS-涂片和痰培养分别是39.29%和37.50%,FQ-PCR法的灵敏度为67.86%,明显高于其他方法。结论 FQ-PCR法和IMS-涂片可作为普通抗酸染色法和培养法的重要补充或是互补的方法,对提高检出率,早发现、早治疗极具临床意义。Objective To perform the comparative analysis on the application value of the clinical diagnostic methods of tuberculosis. Methods The smear, immune magnetic beads technology separation smear（IMS-smear）, sputum culture and FQ PCR meth~,ds were adopted to detect the sputum specimens in 112 cases of tuberculosis and 53 control cases. Results The sensitivity of the common smear method was lowest（25.00%）, which of the IMS- smear and the sputum cuhurc were 39. 29% and 37. 50% respectively. The sensitivity of FQ-PCR was 67. 86%, which was significantly higher than that of other mt.thods. Conclusion FQ-PCR and iMS-smear can be used as an important supplement of common acid fast staining and euhure methods or complementary approach, which has great clinical significance to increase the detection rate,early discover and early treat tuberculosis.

关 键 词：结核分枝杆菌 结核 抗酸染色 免疫磁珠技术 结核培养 荧光定量聚合酶链反应 

分 类 号：R521[医药卫生—内科学]