槲皮素对野百合碱诱导的大鼠肺动脉高压动脉内膜增殖的影响  

Effects of quercetin on proliferation of arterial endothelium of rats with monocrotaline-induced pulmonary arterial hypertension

作　　者：李波[1] 刘映峰[2] 陈灿[3] 黄石安[3] 高汉华[3] 庞玲品[3] 

机构地区：[1]南方医科大学研究生学院,广州510515 [2]南方医科大学珠江医院心血管内科,广州510282 [3]广东医学院附属医院心血管内科二区,广东湛江524001

出　　处：《广东医学》2012年第20期3040-3042,共3页Guangdong Medical Journal

摘　　要：目的观察槲皮素对肺动脉高压动脉内膜增殖的影响。方法将成年雄性SD大鼠30只随机分3组:正常对照组(对照组)、野百合碱(MCT)诱导的肺动脉高压组(MCT组)、槲皮素预防组(预防组),每组10只。测压后处死大鼠,肺组织切片用免疫组化法和免疫印迹法观察大鼠肺血管内皮的增殖Ⅷ因子相关抗原(FⅧ:Ag)情况,并观察肺动脉的形态学变化。结果 MCT组大鼠的FⅧ:Ag显著高于对照组(P<0.05);而预防组大鼠的FⅧ:Ag较MCT组明显降低(P<0.05),但高于对照组(P<0.05)。结论槲皮素可降低MCT所致的肺动脉高压大鼠肺动脉内膜增殖的作用。Objective To investigate the effects of quercetin on the proliferation of arterial endothelium of rats with monocrotaline（MCT） induced pulmonary hypertension.Methods Thirty male SD rats were randomly divided into three groups（n=10）： control group,MCT group and prevention group.The expression of factor Ⅷ：Ag in pulmonary arterial was assessed by immunohistochemistry and Western blot for evaluation of the endodermis proliferation of the pulmonary artery.Meanwhile,the morphologic change in pulmonary artery was observed.Results The expression of factor Ⅷ：Ag was significantly increased in MCT group than that in control group and prevention group（P0.05）.In prevention group,the expression of factor Ⅷ：Ag was also significantly higher than that in control group（P0.05）.Conclusion Quercetin can reduce the MCT-induced endodermis proliferation.

关 键 词：肺动脉高压 槲皮素 内膜增殖 

分 类 号：R87[医药卫生—运动医学]