FSH对胎儿卵巢组织玻璃化冻存及冻融后短时培养的影响  

The Effect of FSH on Cryopreserved Fetal Ovarian Tissue after Short Cultivation

作　　者：王艳盛[1,2] 陈杰[1] 黑长春[1] 王燕蓉[1] 马文智[1] 孙苗[1] 蔡玉芳[1] 王国平 

机构地区：[1]宁夏医科大学人体解剖与组织胚胎学系,宁夏回族自治区生殖与遗传重点实验室,省部共建生殖与遗传教育部重点实验室,银川750004 [2]邢台医学高等专科学校,邢台054000 [3]宁夏银川市妇幼保健院,银川750001

出　　处：《宁夏医科大学学报》2012年第9期869-871,880,F0002,共5页Journal of Ningxia Medical University

基　　金：国家自然科学基金(81160085)

摘　　要：目的通过对FSH干预下的胎儿卵巢玻璃化冷冻及解冻后短暂培养,观察FSH对卵泡储备的影响。方法将20周流产胎儿卵巢皮质切割成体积为5mm×4mm×2mm和10mm×4mm×2mm的皮质片,在玻璃化液、解冻液和培养液中添加10μg FSH.mL-1,通过两步渗透平衡法,将卵巢皮质进行玻璃化冻存。解冻后培养4.5h,通过卵泡形态学观察、不同发育期卵泡构成比和卵泡直径观察评价玻璃化冻存和解冻后的FSH干预下的培养效果。结果①不同发育阶段卵泡构成比及卵泡直径在冻融组之间及冻融组与新鲜组之间相比差异无统计学意义;②冻融组和冻融-培养组之间相比较,培养组卵泡直径增大,差别有统计学意义(P<0.05)。结论冻融过程的FSH添加不影响人胎儿卵巢大皮质片的玻璃化冻存及早期卵泡的构成比,但是体外添加FSH培养4.5h可使一定量的原始卵泡发育至早期初级卵泡阶段。Objective To observe the effects of follicle stimulating hormone （FSH） on reserve of follicles through the adding of FSH during fetal ovary vitrifiation and brief culture. Methods 5mm x4mm x 2mm and 10ram x 4mm x 2mm fragments was prepared with fresh human fetal ovarian cortex from an abortion of 20 weeks , and cryopreserved after two- step osmotic equilibrium added 101μg - mL-1 FSH. After thawing, the ovary fragments were cultured in the medium added 101xg ：mL-1 FSH for 4.5 hours. The follicle diameter and follicle constituent ratio of thawed cultured ovary fragments were detected and compared with the fresh ova- ry fragments. Results ①The constituent ratio of normal follicles and follicular diameter were similar to that in vitrification and thawing groups as compared with fresh control group. ②The follicular diameter of cultivation in thawing groups were longer than that in thawing and culture groups （P 〈 0.05）. Conclusion Adding FSH during vitrifiation and thawing process did not influence constituent ratio of follicles , but some primordial folli- cles could develop to primary follicle after adding FSH in vitro and culturing for 4.5 hours.

关 键 词：胎儿卵巢 玻璃化冻存 FSH 

分 类 号：R321-3[医药卫生—人体解剖和组织胚胎学]