小鼠组织SAM和SAH在动脉粥样硬化评价中的应用  

The Application of SAM and SAH in Mice Tissues in Evaluation of Atherosclerosis

作　　者：梁宇[1] 廖国玲[1] 马琳娜[1] 曹成建[1] 姜怡邓[1] 

机构地区：[1]宁夏医科大学检验学院,银川750004

出　　处：《宁夏医科大学学报》2012年第9期872-875,F0002,共5页Journal of Ningxia Medical University

基　　金：2010国家自然科学基金(30960124);2011国家自然科学基金(81160044);2010年教育部新世纪优秀人才支持计划(NCET-10-0916)

摘　　要：目的探讨S-腺苷同型半胱氨酸(S-adenosyl Homocysteine,SAH)和S-腺苷甲硫氨酸(S-Adenosyl-methionine,SAM)在载脂蛋白E基因敲除(ApoE-/-)小鼠动脉粥样硬化(Atherosclerosis,AS)评价中的应用。方法采用高效液相色谱法(High Performance Liquid Chromatography,HPLC)检测各组ApoE-/-小鼠(模型对照组、高蛋氨酸饮食组、叶酸和维生素B12治疗组)心脏、肝脏、肾脏组织中SAM和SAH含量的变化,并与正常对照组比较。HE染色光镜观察各组主动脉病理学改变。结果 HPLC实验数据表明,正常对照组、模型对照组、高蛋氨酸饮食组的小鼠心脏、肝脏、肾脏组织中SAM、SAH的含量依次增加,治疗组小鼠心脏、肝脏、肾脏组织中SAM、SAH的含量则有所降低,与正常对照组比较差异均有统计学意义(P<0.05)。与模型对照组比较,肾脏高蛋氨酸饮食组SAM、SAH含量差异有统计学意义(P<0.05),治疗组肝脏、肾脏SAM、SAH含量差异有统计学意义(P<0.01)。与高蛋氨酸饮食组比较,肝脏、肾脏治疗组SAM、SAH含量有明显差异(P<0.01)。同时,HE染色显示高蛋氨酸饮食组主动脉AS的病理改变明显。结论 SAM、SAH含量升高可能是高蛋氨酸饮食促进ApoE-/-小鼠动脉粥样硬化发生的重要机制之一。Objective Using high performance liquid chromatography （HPLC） to detect the changes of S - adenosyl- homocysteine（SAH） and S -adenosyl methionine（ SAM）, and to investigate its application in the evaluation of atherosclerosis （AS） in apolipoprotein E - knockout （ ApoE -/- ） mice. Methods SAM and SAH content changes in the hearts, livers and kidneys tissues of ApoE-/- mice groups （model control high -methionine diet group, folic acid and vitamin B12 treatment group） were detected and compared. group, Patho- logical changes of aorta in each group were observed by HE staining with light microscope. Results The re- suits of HPLC showed that SAM, SAH levels in the normal control group, model control group and high - me- thionine diet group miceg hearts, livers and kidneys tissues were increased gradually. The SAM and SAH lev- els in the treatment group of ApoE-/- mice＇s hearts, livers and kidneys tissues were decreased. There were significant differences compared with the normal control group（P 〈 0.05 ）. Compared with the model group, the differences of SAM and SAH contents in kidneys of high methionine diet group were significant （P 〈 0. 05 ）, the contents of SAM and SAH in livers and kidneys of treatment group were significantly different （ P 〈 0.01 ）. Compared with the high methionine diet group, there were significant difference of the SAM and SAH levels in livers, kidneys of treatment group（P 〈0.01 ）. Meanwhile, HE staining showed that AS pathological changes of aorta were significant in high - methionine diet group. Conclusion The increased expression of SAM, SAH may be one of the mechanisms that promoting the occurrence of atherosclerosis in the high - methi- onine diet ApoE -/- mice.

关 键 词：S-腺苷同型半胱氨酸 S-腺苷甲硫氨酸 蛋氨酸 APOE-/-小鼠 

分 类 号：R543.5[医药卫生—心血管疾病]