黄芪汤对肝硬化大鼠肝脏卵圆细胞肝向分化的作用  

Action of astragalus decoction on hepatic oval cells(HOCs) differentiating to liver in cirrhotic rat

作　　者：朱英[1] 刘平[2] 

机构地区：[1]大连医科大学附属第一医院,辽宁大连116011 [2]上海中医药大学

出　　处：《中西医结合肝病杂志》2012年第5期293-295,I0004,共4页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：大连市科学技术局资助(No.2012E15SF143)

摘　　要：目的:研究黄芪汤对肝硬化大鼠肝脏卵圆细胞肝向分化的作用机制。方法:应用二甲基亚硝胺制备大鼠肝硬化模型,用黄芪汤治疗,进行肝功能、肝脏病理学检测;应用共聚焦免疫荧光技术检测肝脏卵圆细胞与肝细胞及胆管细胞共定位染色。结果:黄芪汤能显著降低模型大鼠血清AST、TBil水平和肝组织Hyp含量,减少肝组织α-SMA的表达,在模型大鼠肝纤维化逆转过程中,黄芪汤可使Thy1.1与CK19共定位细胞数量显著增加,使肝脏卵圆细胞(HOC)表型和功能发生改变。结论:黄芪汤通过诱导肝脏卵圆细胞肝向分化作用来促进肝硬化逆转。Objective：To study the action mechanism of astragalus decoction on hepatic oval cells（HOCs） differentiating to liver in cirrhotic rat.Methods：The rat liver cirrhosis model was prepared with dimethylnitrosamine,was treated with astragalus decoction.Their liver function and liver pathology,were observed HOCs and hepatocytes and bile duct cells colocalized staining were deterted with confocal immunofluorescence.Results： In the course of cirrhosis reversion,astragalus decoction significantly reduced the serum AST,TBil and liver tissue Hyp content,reducing the expression of α-SMA in liver tissue,astragalus decoction can also increased co-localization cell number of Thy1.1 and CK19 significantly,making the HOCs phenotypic and functional changed.Conclusion：Astragalus decoction can promote cirrhosis reversal through the induction of HOCs differentiating to liver.

关 键 词：黄芪汤 肝硬化 卵圆细胞 肝脏 大鼠 

分 类 号：R285.5[医药卫生—中药学]