新疆大花红景天化学成分的HPLC-MS研究  

Analysis of Chemical Constituents of Rhodiola crenulata Using High Performance Liquid Chromatography-Mass Spectrometry

作　　者：霍佳丽[1] 王建华[1] 吴志军[2] 张国林[2] 

机构地区：[1]重庆大学生物工程学院,重庆400044 [2]中国科学院成都生物研究所,成都610041

出　　处：《天然产物研究与开发》2012年第10期1405-1407,共3页Natural Product Research and Development

基　　金：国家科技支撑计划(2007BAD51B05)

摘　　要：采用高效液相色谱-电喷雾-四极杆-飞行时间串联质谱法(HPLC-ESI-Q-TOF-MS),分别在正负离子模式下对新疆产大花红景天(Rhodiola crenulata)提取物进行分离分析。通过精确质量测定和同位素峰形匹配(Sig-maFit)确定了化合物的元素组成。在正离子模式下,选择[M+H]+或[M+NH4]+准分子离子进行二级裂解对其结构进行分析。根据物质的元素组成和二级质谱信息,初步确定了新疆大花红景天提取物中的12种化合物。The extracts of Rhodiola crenulata in Xinjiang were investigated by high-performance liquid chromatography and electrospray quadrupole time-of-flight mass spectrometry （HPLC-ESI-Q-TOF-MS） in both positive- and negative-ion mode. Elemental compositions of compounds were confirmed by accurate mass and isotope analysis （the value of SigmaFit）. Tandem MS was further applied for the structural analysis in positive-ion mode, and protonated or NH4＋ adduct precursor ions were selected for the fragmentation experiments. Twelve compounds, including a pair of isomer, from the extracts of R. crenulata were identified or tentatively characterized based on elemental compositions and tandem mass spectra.

关 键 词：新疆大花红景天 液质联用(HPLC-MS) 红景天苷 

分 类 号：R284.2[医药卫生—中药学]